Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA3-026 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CABIN1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Cain, a novel physiologic protein inhibitor of calcineurin.
Lai MM, Burnett PE, Wolosker H, Blackshaw S, Snyder SH
The Journal of biological chemistry 1998 Jul 17;273(29):18325-31
The Journal of biological chemistry 1998 Jul 17;273(29):18325-31
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunofluorescent analysis of CABIN1 (green) in NRK cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a CABIN1 antibody (Product # PA3-026) at a dilution of 20 µg/mL in staining buffer for 1 hour at room temperature, and then incubated with a Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor 488 conjugate (Product # A27034) at a dilution of 1:1000 for 1 hour at room temperature (green). Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.