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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [6]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-27484 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ILK Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, A431, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12.
- Concentration
- 0.8 mg/mL
Submitted references Enhancement of aberrantly modified integrin‑mediated cell motility in multicellular tumor spheroids.
Kim B, Im NR, Yang TD, Kim J, Jung KY, Kim TH, Baek SK
International journal of oncology 2020 Jun;56(6):1490-1498
International journal of oncology 2020 Jun;56(6):1490-1498
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ILK was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a ILK Polyclonal Antibody (Product # PA5-27484) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ILK was performed by separating 30 µg of whole cell extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a ILK Polyclonal Antibody (Product # PA5-27484) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ILK was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a ILK Polyclonal Antibody (Product # PA5-27484) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ILK Polyclonal Antibody (Product # PA5-27484). Sample (30 µg of whole cell lysate). A: A431. 7.5% SDS PAGE. ILK Polyclonal Antibody (Product # PA5-27484) diluted at 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ILK Polyclonal Antibody detects ILK protein by western blot analysis. A. 30 µg Neuro2A whole cell extract. B. 30 µg C8D30 whole cell extract. C. 30 µg NIH-3T3 whole cell extract. D. 30 µg Raw 264.7 whole cell extract. E. 30 µg C2Cl2 whole cell extract.10 % SDS-PAGE. ILK Polyclonal Antibody (Product # PA5-27484) dilution: 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ILK was performed by separating 30 µg of whole cell extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a ILK Polyclonal Antibody (Product # PA5-27484) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of ILK was performed in paraffin-embedded mouse kidney tissue using ILK Polyclonal Antibody (Product # PA5-27484) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ILK Polyclonal Antibody immunoprecipitates ILK protein in IP experiments. IP samples: HeLa whole cell extract. A. 30 µg HeLa whole cell extract. B. Control with 4 µg of preimmune Rabbit IgG. C. Immunoprecipitation of ILK protein by 4 µg ILK Polyclonal Antibody (Product # PA5-27484). 10 % SDS-PAGE. The immunoprecipitated ILK protein was detected by ILK Polyclonal Antibody (Product # PA5-27484) diluted at 1:500.
