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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [2]
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- Product number
- PA5-30848 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SETDB2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: SK-N-SH.
- Concentration
- 1 mg/mL
Submitted references SETDB2 promoted breast cancer stem cell maintenance by interaction with and stabilization of ΔNp63α protein.
Ying L, Fei X, Jialun L, Jianpeng X, Jie W, Zhaolin M, Hongjia F, Huan F, Sha L, Qiuju W, Lin Y, Cuicui L, You P, Weiwei Z, Lulu W, Jiemin W, Jing L, Jing F
International journal of biological sciences 2020;16(12):2180-2191
International journal of biological sciences 2020;16(12):2180-2191
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SETDB2 Polyclonal Antibody (Product # PA5-30848). Sample (30 µg of whole cell lysate). Lane A: SK-N-SH . 7.5% SDS PAGE. SETDB2 Polyclonal Antibody (Product # PA5-30848) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SETDB2 Polyclonal Antibody detects SETDB2 protein at nucleus by immunofluorescent analysis. Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SETDB2 protein stained by SETDB2 Polyclonal Antibody (Product # PA5-30848) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SETDB2 antibody immunoprecipitates SETDB2 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 µg HeLa whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of SETDB2 protein by 2 µg of SETDB2 antibody (Product # PA5-30848) 7.5% SDS-PAGE The immunoprecipitated SETDB2 protein was detected by SETDB2 antibody (Product # PA5-30848) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 SETDB2 interacted with DeltaNp63alpha, and SETDB2 silencing downregulated Hedgehog pathway-associated genes and the DeltaNp63alpha protein level. ( A ) Heat map analysis of RNA-seq results in SETDB2-knockdown SUM159PT cells. ( B ) The mRNA levels of SETDB2 and Hedgehog pathway-associated genes ( CXCR4 , PTCH1 and GLI2 ) were analyzed by Q-PCR in SETDB2-knockdown SUM159PT and MDA-MB-231 cells. ( C ) The mRNA levels of Hedgehog pathway-associated genes ( CXCR4 , PTCH1 and GLI2 ) were analyzed by Q-PCR in NC, SETDB2-knockdown and SETDB2-rescued SUM159PT cells. ( D ) The protein levels of SETDB2 and DeltaNp63alpha were analyzed by Western blot in SETDB2-knockdown SUM159PT and MDA-MB-231 cells. A GAPDH antibody was used as a loading control. ( E ) The level of DeltaNp63alpha mRNA was analyzed by RT-PCR in SETDB2-knockdown SUM159PT and MDA-MB-231 cells. ( F ) Immunoprecipitation of SETDB2 showed that DeltaNp63alpha endogenously interacted with SETDB2 in SUM159PT and MDA-MB-231 cells. ( G ) The interaction between exogenous Flag-SETDB2 and endogenous DeltaNp63alpha were analyzed by anti-flag antibody immunoprecipitation in SETDB2-rescued SUM159PT cells. ( H ) SETDB2 and DeltaNp63alpha were co-expressed in 293T cells. The exogenous interactions between SETDB2 and DeltaNp63alpha were analyzed by co-immunoprecipitation and Western blotting. (NC: Negative Control; shS-3: shSETDB2-3; shS-4: shSETDB2-4; data are presented as the mean +- SD of three independent experiments. *** P
