NB100-1137

antibody from Novus Biologicals

Targeting: SETDB2 C13orf4, CLLD8, CLLL8, KMT1F

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-1137

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-1137, RRID:AB_10000521

Product name

Goat Polyclonal SETDB2 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. This antibody is expected to recognize both reported isoforms (NP_114121.2

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

0.1 mg

Concentration

0.5 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

References

Submitted references

IFI16, a nuclear innate immune DNA sensor, mediates epigenetic silencing of herpesvirus genomes by its association with H3K9 methyltransferases SUV39H1 and GLP.

Roy A, Ghosh A, Kumar B, Chandran B
eLife 2019 Nov 4;8

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Cloning and characterization of CLLD6, CLLD7, and CLLD8, novel candidate genes for leukemogenesis at chromosome 13q14, a region commonly deleted in B-cell chronic lymphocytic leukemia.

Mabuchi H, Fujii H, Calin G, Alder H, Negrini M, Rassenti L, Kipps TJ, Bullrich F, Croce CM
Cancer research 2001 Apr 1;61(7):2870-7

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: SETDB2 Antibody [NB100-1137] - RL human non-Hodgkin's lymphoma B cell line whole cell lysate (WCL). Single band around correct MW is specific to the target protein SETDB2. Image submitted by a verified customer review.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: SETDB2 Antibody [NB100-1137] - Staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Chromatin Immunoprecipitation (ChIP): SETDB2 Antibody [NB100-1137] - Human B-cell nuclear extract (500 ug). Gene of interest promoter primers were used for amplification to detect ChIP efficiency to IgG control. Image submitted by a verified customer review.