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- Antibody Data
- Antigen structure
- References [8]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- 10334-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#10334-1-AP, RRID:AB_2211314
- Product name
- U2AF35 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated U2AF35 antibody (Cat. #10334-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IHC, WB, ELISA.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Splicing Enhancers at Intron-Exon Borders Participate in Acceptor Splice Sites Recognition.
PHF5A Epigenetically Inhibits Apoptosis to Promote Breast Cancer Progression.
PUF60-activated exons uncover altered 3' splice-site selection by germline missense mutations in a single RRM.
U2AF35(S34F) Promotes Transformation by Directing Aberrant ATG7 Pre-mRNA 3' End Formation.
Identification of U2AF(35)-dependent exons by RNA-Seq reveals a link between 3' splice-site organization and activity of U2AF-related proteins.
Resveratrol, by modulating RNA processing factor levels, can influence the alternative splicing of pre-mRNAs.
Allele-specific recognition of the 3' splice site of INS intron 1.
Splicing factor SPF30 bridges an interaction between the prespliceosome protein U2AF35 and tri-small nuclear ribonucleoprotein protein hPrp3.
Kováčová T, Souček P, Hujová P, Freiberger T, Grodecká L
International journal of molecular sciences 2020 Sep 8;21(18)
International journal of molecular sciences 2020 Sep 8;21(18)
PHF5A Epigenetically Inhibits Apoptosis to Promote Breast Cancer Progression.
Zheng YZ, Xue MZ, Shen HJ, Li XG, Ma D, Gong Y, Liu YR, Qiao F, Xie HY, Lian B, Sun WL, Zhao HY, Yao L, Zuo WJ, Li DQ, Wang P, Hu X, Shao ZM
Cancer research 2018 Jun 15;78(12):3190-3206
Cancer research 2018 Jun 15;78(12):3190-3206
PUF60-activated exons uncover altered 3' splice-site selection by germline missense mutations in a single RRM.
Královicová J, Ševcíková I, Stejskalová E, Obuca M, Hiller M, Stanek D, Vorechovský I
Nucleic acids research 2018 Jul 6;46(12):6166-6187
Nucleic acids research 2018 Jul 6;46(12):6166-6187
U2AF35(S34F) Promotes Transformation by Directing Aberrant ATG7 Pre-mRNA 3' End Formation.
Park SM, Ou J, Chamberlain L, Simone TM, Yang H, Virbasius CM, Ali AM, Zhu LJ, Mukherjee S, Raza A, Green MR
Molecular cell 2016 May 19;62(4):479-90
Molecular cell 2016 May 19;62(4):479-90
Identification of U2AF(35)-dependent exons by RNA-Seq reveals a link between 3' splice-site organization and activity of U2AF-related proteins.
Kralovicova J, Knut M, Cross NC, Vorechovsky I
Nucleic acids research 2015 Apr 20;43(7):3747-63
Nucleic acids research 2015 Apr 20;43(7):3747-63
Resveratrol, by modulating RNA processing factor levels, can influence the alternative splicing of pre-mRNAs.
Markus MA, Marques FZ, Morris BJ
PloS one 2011;6(12):e28926
PloS one 2011;6(12):e28926
Allele-specific recognition of the 3' splice site of INS intron 1.
Kralovicova J, Vorechovsky I
Human genetics 2010 Oct;128(4):383-400
Human genetics 2010 Oct;128(4):383-400
Splicing factor SPF30 bridges an interaction between the prespliceosome protein U2AF35 and tri-small nuclear ribonucleoprotein protein hPrp3.
Little JT, Jurica MS
The Journal of biological chemistry 2008 Mar 28;283(13):8145-52
The Journal of biological chemistry 2008 Mar 28;283(13):8145-52
No comments: Submit comment
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- COLO 320 cells were subjected to SDS PAGE followed by western blot with 10334-1-AP(U2AF35 antibody) at dilution of 1:1000
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human prostate cancer using 10334-1-AP(U2AF35 antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The U2AF35 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human U2AF35. This antibody recognizes human, mouse, rat antigen. The U2AF35 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
