F54776

antibody from NSJ Bioreagents

Targeting: U2AF1 RN, RNU2AF1, U2AF35, U2AFBP

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54776

Provider

NSJ Bioreagents

Product name

U2AF1 Antibody / U2AF35

Antibody type

Polyclonal

Description

Purified

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 µl, 0.4 µl

Storage

Aliquot the U2AF1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of Ramos cell lysate with U2AF1 antibody. Expected molecular weight: 28-35 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with U2AF1 antibody. Expected molecular weight: 28-35 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human lung carcinoma tissue with U2AF1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human Ramos cells with U2AF1 antibody; Blue=isotype control, Green= U2AF1 antibody.