60289-1-IG

antibody from Invitrogen Antibodies

Targeting: U2AF1 RN, RNU2AF1, U2AF35, U2AFBP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

60289-1-IG

Provider

Invitrogen Antibodies

Product name

U2AF35 Monoclonal Antibody (2B7F11)

Antibody type

Monoclonal

Antigen

Other

Description

Immunogen sequence: ACRHGDRCS RLHNKPTFSQ TIALLNIYRN PQNSSQSADG LRCAVSDVEM QEHYDEFFEE VFTEMEEKYG EVEEMNVCDN LGDHLVGNVY VKFRREEDAE KAVIDLNNRW FNGQPIHAEL SPVTDFREAC CRQYEMGECT RGGFCNFMHL KPISRELRRE LYGRRRKKHR SRSRSRERRS RSRDRGRGGG GGGGGGGGGR ERDRRRSRDR ERSGRF (26-240 aa encoded by BC001923)

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

2B7F11

Vial size

150 µL

Concentration

1.11 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 60289-1-IG ( U2AF35 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

COLO 320 cells were subjected to SDS PAGE followed by western blot with 60289-1-Ig (U2AF35 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 4% PFA) fixed HepG2 cells using 60289-1-IG (U2AF35 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 60289-1-IG ( U2AF35 Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 60289-1-Ig (U2AF35 Antibody) at dilution of 1:50 (under 10x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-U2AF35 (IP:60289-1-IG, 3ug; Detection:60289-1-IG 1:500) with HeLa cells lysate 3000ug.