GTX105620

antibody from GeneTex

Targeting: AKR1C1 DD1, DDH, DDH1, HAKRC, MBAB

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX105620

Provider

GeneTex

Proper citation

GeneTex Cat#GTX105620, RRID:AB_1949621

Product name

AKR1C1 antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

References

Submitted references

Preferential expression of potential markers for cancer stem cells in large cell neuroendocrine carcinoma of the lung. An FFPE proteomic study.

Nomura M, Fukuda T, Fujii K, Kawamura T, Tojo H, Kihara M, Bando Y, Gazdar AF, Tsuboi M, Oshiro H, Nagao T, Ohira T, Ikeda N, Gotoh N, Kato H, Marko-Varga G, Nishimura T
Journal of clinical bioinformatics 2011 Sep 3;1(1):23

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample(30 ?g whole cell lysate)A:Raji (GTX27908)10% SDS PAGEGTX105620 diluted at 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

AKR1C1 antibody detects AKR1C1 protein by western blot analysis.A. 50 ?g mouse liver lysate/extract10% SDS-PAGEAKR1C1 antibody (GTX105620) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with AKR1C1 antibody (GTX105620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE GTX105620 diluted at 1:1000

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescence analysis of methanol-fixed A431, using AKR1C1(GTX105620) antibody at 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft , using AKR1C1(GTX105620) antibody at 1:500 dilution.