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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [6]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-21672 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AKR1C1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, HeLa, HepG2, A549, mouse liver.
- Concentration
- 0.6 mg/mL
Submitted references Regulation of uterine function during estrous cycle, anestrus phase and pregnancy by steroids in red deer (Cervus elaphus L.).
Kotlarczyk AM, Grzyb M, Korzekwa AJ
Scientific reports 2021 Oct 11;11(1):20109
Scientific reports 2021 Oct 11;11(1):20109
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of AKR1C1 using 30 µg of Raji lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an AKR1C1 polyclonal antibody (Product # PA5-21672) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of AKR1C1 in Various whole cell extracts (30 µg). Samples were separated by 10% SDS-PAGE and the membrane was probed with AKR1C1 Polyclonal antibody (Product # PA5-21672) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of AKR1C1 was performed by separating 30 µg of A549 lysates by 10% SDS PAGE. Proteins were transferred to a membrane and probed with a AKR1C1 Polyclonal Antibody (Product # PA5-21672) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- AKR1C1 Polyclonal Antibody detects AKR1C1 protein by western blot analysis. A. 50 µg mouse liver lysate/extract.10% SDS-PAGE. AKR1C1 Polyclonal Antibody (Product # PA5-21672) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of AKR1C1 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a AKR1C1 Polyclonal Antibody (Product # PA5-21672) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of AKR1C1 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a AKR1C1 Polyclonal Antibody (Product # PA5-21672) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of AKR1C1 in methanol-fixed A431 cells using an AKR1C1 polyclonal antibody (Product # PA5-21672) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed A431, using AKR1C1 antibody (Product # PA5-21672) at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft , using AKR1C1 (Product # PA5-21672) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 mRNA and protein expression of AKR1C1 ( A , E ), P450 ( B , F ), 3beta-HSD ( C , G ) and 17beta-HSD ( D , H ) in uterine tissues (endometrium and myometrium) on 4th and 13th day of estrous cycle, in pregnancy and anestrus phase. Data were normalized against GAPDH for mRNA expression and against beta-actin (ACTB) for proteins expression. Each bar represents one experimental group with SEM. Statistical differences were analyzed by two-way analysis (ANOVA) of variance followed by the Bonferroni post hoc test using GraphPad PRISM (Version 8.3.0). The lowest statistical significance was P < 0.05. Asterisks indicate statistical differences between endometrium and myometrium (* P < 0.1; ** P < 0.01; *** P < 0.001; **** P < 0.0001). Different letters indicate statistical differences ( P < 0.05) between the experimental groups throughout endometrium (A, B) and myometrium (a-b) respectively.
