Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- 66191-1-Ig - Provider product page
- Provider
- Proteintech Group
- Product name
- VAPB antibody
- Antibody type
- Monoclonal
- Description
- KD/KO validated VAPB antibody (Cat. #66191-1-Ig) is a mouse monoclonal antibody that shows reactivity with human and has been validated for the following applications: IF, IHC, WB, ELISA.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 4F6A6
- Vial size
- 20ul, 150ul
Submitted references Atlastin 2/3 regulate ER targeting of the ULK1 complex to initiate autophagy.
CALCOCO1 acts with VAMP-associated proteins to mediate ER-phagy.
Proteomic mapping by rapamycin-dependent targeting of APEX2 identifies binding partners of VAPB at the inner nuclear membrane.
Fluorescent tools to analyse peroxisome-ER interactions in mammalian cells.
Host Vesicle Fusion Protein VAPB Contributes to the Nuclear Egress Stage of Herpes Simplex Virus Type-1 (HSV-1) Replication.
The ER Contact Proteins VAPA/B Interact with Multiple Autophagy Proteins to Modulate Autophagosome Biogenesis.
Liu N, Zhao H, Zhao YG, Hu J, Zhang H
The Journal of cell biology 2021 Jul 5;220(7)
The Journal of cell biology 2021 Jul 5;220(7)
CALCOCO1 acts with VAMP-associated proteins to mediate ER-phagy.
Nthiga TM, Kumar Shrestha B, Sjøttem E, Bruun JA, Bowitz Larsen K, Bhujabal Z, Lamark T, Johansen T
The EMBO journal 2020 Aug 3;39(15):e103649
The EMBO journal 2020 Aug 3;39(15):e103649
Proteomic mapping by rapamycin-dependent targeting of APEX2 identifies binding partners of VAPB at the inner nuclear membrane.
James C, Müller M, Goldberg MW, Lenz C, Urlaub H, Kehlenbach RH
The Journal of biological chemistry 2019 Nov 1;294(44):16241-16254
The Journal of biological chemistry 2019 Nov 1;294(44):16241-16254
Fluorescent tools to analyse peroxisome-ER interactions in mammalian cells.
Bishop A, Kamoshita M, Passmore JB, Hacker C, Schrader TA, Waterham HR, Costello JL, Schrader M
Contact (Thousand Oaks (Ventura County, Calif.)) 2019 Jun 5;2
Contact (Thousand Oaks (Ventura County, Calif.)) 2019 Jun 5;2
Host Vesicle Fusion Protein VAPB Contributes to the Nuclear Egress Stage of Herpes Simplex Virus Type-1 (HSV-1) Replication.
Saiz-Ros N, Czapiewski R, Epifano I, Stevenson A, Swanson SK, Dixon CR, Zamora DB, McElwee M, Vijayakrishnan S, Richardson CA, Dong L, Kelly DA, Pytowski L, Goldberg MW, Florens L, Graham SV, Schirmer EC
Cells 2019 Feb 3;8(2)
Cells 2019 Feb 3;8(2)
The ER Contact Proteins VAPA/B Interact with Multiple Autophagy Proteins to Modulate Autophagosome Biogenesis.
Zhao YG, Liu N, Miao G, Chen Y, Zhao H, Zhang H
Current biology : CB 2018 Apr 23;28(8):1234-1245.e4
Current biology : CB 2018 Apr 23;28(8):1234-1245.e4
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 66191-1-Ig( VAPB Antibody) at dilution of 1:1000
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The VAPB antibody from Proteintech is a mouse monoclonal antibody to a recombinant protein of human VAPB. This antibody recognizes human antigen. The VAPB antibody has been validated for the following applications: ELISA, WB, IF, IHC analysis.