Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [38]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 14477-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#14477-1-AP, RRID:AB_2288297
- Product name
- VAPB antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated VAPB antibody (Cat. #14477-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references CGI1746 targets σ(1)R to modulate ferroptosis through mitochondria-associated membranes.
ARTC1-mediated VAPB ADP-ribosylation regulates calcium homeostasis.
VAP spatially stabilizes dendritic mitochondria to locally support synaptic plasticity.
Development of a signal-integrating reporter to monitor mitochondria-ER contacts.
USP20 deubiquitinates and stabilizes the reticulophagy receptor RETREG1/FAM134B to drive reticulophagy.
Pathological convergence of APP and SNCA deficiency in hippocampal degeneration of young rats.
A SNX1-SNX2-VAPB partnership regulates endosomal membrane rewiring in response to nutritional stress.
Deficiency of Perry syndrome-associated p150(Glued) in midbrain dopaminergic neurons leads to progressive neurodegeneration and endoplasmic reticulum abnormalities.
Elucidation of OSW-1-Induced Stress Responses in Neuro2a Cells.
Oligomeric CHMP7 mediates three-way ER junctions and ER-mitochondria interactions.
The p97-UBXD8 complex regulates ER-Mitochondria contact sites by altering membrane lipid saturation and composition.
The novel bacterial effector protein CbEPF1 mediates ER-LD membrane contacts to regulate host lipid droplet metabolism.
ER-mitochondria contacts and cholesterol metabolism are disrupted by disease-associated tau protein.
Deregulation of ER-mitochondria contact formation and mitochondrial calcium homeostasis mediated by VDAC in fragile X syndrome.
SPTLC1 variants associated with ALS produce distinct sphingolipid signatures through impaired interaction with ORMDL proteins.
Proteomic Mapping and Targeting of Mitotic Pericentriolar Material in Tumors Bearing Centrosome Amplification.
Serine palmitoyltransferase assembles at ER-mitochondria contact sites.
Mobile late endosomes modulate peripheral endoplasmic reticulum network architecture.
The role of Mfn2 in the structure and function of endoplasmic reticulum-mitochondrial tethering in vivo.
The Novel ALG-2 Target Protein CDIP1 Promotes Cell Death by Interacting with ESCRT-I and VAPA/B.
MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.
VPS13D interacts with VCP/p97 and negatively regulates endoplasmic reticulum-mitochondria interactions.
A High-Density Human Mitochondrial Proximity Interaction Network.
Proteomics-Based Approach Identifies Altered ER Domain Properties by ALS-Linked VAPB Mutation.
Nir2 Is an Effector of VAPs Necessary for Efficient Hepatitis C Virus Replication and Phosphatidylinositol 4-Phosphate Enrichment at the Viral Replication Organelle.
A trap mutant reveals the physiological client spectrum of TRC40.
MIGA2 Links Mitochondria, the ER, and Lipid Droplets and Promotes De Novo Lipogenesis in Adipocytes.
Long-chain acyl-CoA synthetase 1 interacts with key proteins that activate and direct fatty acids into niche hepatic pathways.
Loss of VAPB Regulates Autophagy in a Beclin 1-Dependent Manner.
DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.
Sequence-dependent cargo recognition by SNX-BARs mediates retromer-independent transport of CI-MPR.
Adenovirus Modulates Toll-Like Receptor 4 Signaling by Reprogramming ORP1L-VAP Protein Contacts for Cholesterol Transport from Endosomes to the Endoplasmic Reticulum.
Neuronal overexpression of human VAPB slows motor impairment and neuromuscular denervation in a mouse model of ALS.
The ATF6 pathway of the ER stress response contributes to enhanced viability in glioblastoma.
The role of endoplasmic reticulum-related BiP/GRP78 in interferon gamma-induced persistent Chlamydia pneumoniae infection.
Expression of vesicle-associated membrane-protein-associated protein B cleavage products in peripheral blood leukocytes and cerebrospinal fluid of patients with sporadic amyotrophic lateral sclerosis.
Widespread aggregation of mutant VAPB associated with ALS does not cause motor neuron degeneration or modulate mutant SOD1 aggregation and toxicity in mice.
Disease mutations in Rab7 result in unregulated nucleotide exchange and inappropriate activation.
Zhang Z, Zhou H, Gu W, Wei Y, Mou S, Wang Y, Zhang J, Zhong Q
Nature chemical biology 2024 Jun;20(6):699-709
Nature chemical biology 2024 Jun;20(6):699-709
ARTC1-mediated VAPB ADP-ribosylation regulates calcium homeostasis.
Ma X, Li M, Liu Y, Zhang X, Yang X, Wang Y, Li Y, Wang J, Liu X, Yan Z, Yu X, Wu C
Journal of molecular cell biology 2024 Jan 5;15(7)
Journal of molecular cell biology 2024 Jan 5;15(7)
VAP spatially stabilizes dendritic mitochondria to locally support synaptic plasticity.
Bapat O, Purimetla T, Kruessel S, Shah M, Fan R, Thum C, Rupprecht F, Langer JD, Rangaraju V
Nature communications 2024 Jan 4;15(1):205
Nature communications 2024 Jan 4;15(1):205
Development of a signal-integrating reporter to monitor mitochondria-ER contacts.
Yang Z, Chan DC
bioRxiv : the preprint server for biology 2024 Feb 14;
bioRxiv : the preprint server for biology 2024 Feb 14;
USP20 deubiquitinates and stabilizes the reticulophagy receptor RETREG1/FAM134B to drive reticulophagy.
Zhang M, Wang Z, Zhao Q, Yang Q, Bai J, Yang C, Zhang ZR, Liu Y
Autophagy 2024 Aug;20(8):1780-1797
Autophagy 2024 Aug;20(8):1780-1797
Pathological convergence of APP and SNCA deficiency in hippocampal degeneration of young rats.
Wang Y, Miao Z, Xu C, Cai Y, Yang Y, Hu Y, Zhao M, Shao Y, Li Z, Chen J, Chen S, Wang L
Cell death & disease 2023 May 13;14(5):325
Cell death & disease 2023 May 13;14(5):325
A SNX1-SNX2-VAPB partnership regulates endosomal membrane rewiring in response to nutritional stress.
Da Graça J, Charles J, Djebar M, Alvarez-Valadez K, Botti J, Morel E
Life science alliance 2023 Mar;6(3)
Life science alliance 2023 Mar;6(3)
Deficiency of Perry syndrome-associated p150(Glued) in midbrain dopaminergic neurons leads to progressive neurodegeneration and endoplasmic reticulum abnormalities.
Yu J, Yang X, Zheng J, Sgobio C, Sun L, Cai H
NPJ Parkinson's disease 2023 Mar 7;9(1):35
NPJ Parkinson's disease 2023 Mar 7;9(1):35
Elucidation of OSW-1-Induced Stress Responses in Neuro2a Cells.
Oh-Hashi K, Nakamura H, Ogawa H, Hirata Y, Sakurai K
International journal of molecular sciences 2023 Mar 17;24(6)
International journal of molecular sciences 2023 Mar 17;24(6)
Oligomeric CHMP7 mediates three-way ER junctions and ER-mitochondria interactions.
Chu Q, Wang J, Du Y, Zhou T, Shi A, Xiong J, Ji WK, Deng L
Cell death and differentiation 2023 Jan;30(1):94-110
Cell death and differentiation 2023 Jan;30(1):94-110
The p97-UBXD8 complex regulates ER-Mitochondria contact sites by altering membrane lipid saturation and composition.
Ganji R, Paulo JA, Xi Y, Kline I, Zhu J, Clemen CS, Weihl CC, Purdy JG, Gygi SP, Raman M
Nature communications 2023 Feb 6;14(1):638
Nature communications 2023 Feb 6;14(1):638
The novel bacterial effector protein CbEPF1 mediates ER-LD membrane contacts to regulate host lipid droplet metabolism.
Angara RK, Sadi A, Gilk SD
bioRxiv : the preprint server for biology 2023 Dec 11;
bioRxiv : the preprint server for biology 2023 Dec 11;
ER-mitochondria contacts and cholesterol metabolism are disrupted by disease-associated tau protein.
Szabo L, Cummins N, Paganetti P, Odermatt A, Papassotiropoulos A, Karch C, Götz J, Eckert A, Grimm A
EMBO reports 2023 Aug 3;24(8):e57499
EMBO reports 2023 Aug 3;24(8):e57499
Deregulation of ER-mitochondria contact formation and mitochondrial calcium homeostasis mediated by VDAC in fragile X syndrome.
Geng J, Khaket TP, Pan J, Li W, Zhang Y, Ping Y, Cobos Sillero MI, Lu B
Developmental cell 2023 Apr 10;58(7):597-615.e10
Developmental cell 2023 Apr 10;58(7):597-615.e10
SPTLC1 variants associated with ALS produce distinct sphingolipid signatures through impaired interaction with ORMDL proteins.
Lone MA, Aaltonen MJ, Zidell A, Pedro HF, Morales Saute JA, Mathew S, Mohassel P, Bönnemann CG, Shoubridge EA, Hornemann T
The Journal of clinical investigation 2022 Sep 15;132(18)
The Journal of clinical investigation 2022 Sep 15;132(18)
Proteomic Mapping and Targeting of Mitotic Pericentriolar Material in Tumors Bearing Centrosome Amplification.
Xie B, Pu Y, Yang F, Chen W, Yue W, Ma J, Zhang N, Jiang Y, Wu J, Lin Y, Liang X, Wang C, Zou P, Li M
Cancer research 2022 Jul 18;82(14):2576-2592
Cancer research 2022 Jul 18;82(14):2576-2592
Serine palmitoyltransferase assembles at ER-mitochondria contact sites.
Aaltonen MJ, Alecu I, König T, Bennett SA, Shoubridge EA
Life science alliance 2022 Feb;5(2)
Life science alliance 2022 Feb;5(2)
Mobile late endosomes modulate peripheral endoplasmic reticulum network architecture.
Spits M, Heesterbeek IT, Voortman LM, Akkermans JJ, Wijdeven RH, Cabukusta B, Neefjes J
EMBO reports 2021 Mar 3;22(3):e50815
EMBO reports 2021 Mar 3;22(3):e50815
The role of Mfn2 in the structure and function of endoplasmic reticulum-mitochondrial tethering in vivo.
Han S, Zhao F, Hsia J, Ma X, Liu Y, Torres S, Fujioka H, Zhu X
Journal of cell science 2021 Jul 1;134(13)
Journal of cell science 2021 Jul 1;134(13)
The Novel ALG-2 Target Protein CDIP1 Promotes Cell Death by Interacting with ESCRT-I and VAPA/B.
Inukai R, Mori K, Kuwata K, Suzuki C, Maki M, Takahara T, Shibata H
International journal of molecular sciences 2021 Jan 25;22(3)
International journal of molecular sciences 2021 Jan 25;22(3)
MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.
König T, Nolte H, Aaltonen MJ, Tatsuta T, Krols M, Stroh T, Langer T, McBride HM
Nature cell biology 2021 Dec;23(12):1271-1286
Nature cell biology 2021 Dec;23(12):1271-1286
VPS13D interacts with VCP/p97 and negatively regulates endoplasmic reticulum-mitochondria interactions.
Du Y, Wang J, Xiong J, Fang N, Ji WK
Molecular biology of the cell 2021 Aug 1;32(16):1474-1486
Molecular biology of the cell 2021 Aug 1;32(16):1474-1486
A High-Density Human Mitochondrial Proximity Interaction Network.
Antonicka H, Lin ZY, Janer A, Aaltonen MJ, Weraarpachai W, Gingras AC, Shoubridge EA
Cell metabolism 2020 Sep 1;32(3):479-497.e9
Cell metabolism 2020 Sep 1;32(3):479-497.e9
Proteomics-Based Approach Identifies Altered ER Domain Properties by ALS-Linked VAPB Mutation.
Yamanaka T, Nishiyama R, Shimogori T, Nukina N
Scientific reports 2020 May 6;10(1):7610
Scientific reports 2020 May 6;10(1):7610
Nir2 Is an Effector of VAPs Necessary for Efficient Hepatitis C Virus Replication and Phosphatidylinositol 4-Phosphate Enrichment at the Viral Replication Organelle.
Wang H, Tai AW
Journal of virology 2019 Nov 15;93(22)
Journal of virology 2019 Nov 15;93(22)
A trap mutant reveals the physiological client spectrum of TRC40.
Coy-Vergara J, Rivera-Monroy J, Urlaub H, Lenz C, Schwappach B
Journal of cell science 2019 Jul 1;132(13)
Journal of cell science 2019 Jul 1;132(13)
MIGA2 Links Mitochondria, the ER, and Lipid Droplets and Promotes De Novo Lipogenesis in Adipocytes.
Freyre CAC, Rauher PC, Ejsing CS, Klemm RW
Molecular cell 2019 Dec 5;76(5):811-825.e14
Molecular cell 2019 Dec 5;76(5):811-825.e14
Long-chain acyl-CoA synthetase 1 interacts with key proteins that activate and direct fatty acids into niche hepatic pathways.
Young PA, Senkal CE, Suchanek AL, Grevengoed TJ, Lin DD, Zhao L, Crunk AE, Klett EL, Füllekrug J, Obeid LM, Coleman RA
The Journal of biological chemistry 2018 Oct 26;293(43):16724-16740
The Journal of biological chemistry 2018 Oct 26;293(43):16724-16740
Loss of VAPB Regulates Autophagy in a Beclin 1-Dependent Manner.
Wu D, Hao Z, Ren H, Wang G
Neuroscience bulletin 2018 Dec;34(6):1037-1046
Neuroscience bulletin 2018 Dec;34(6):1037-1046
DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.
Zheng P, Chen Q, Tian X, Qian N, Chai P, Liu B, Hu J, Blackstone C, Zhu D, Teng J, Chen J
Cell research 2018 Aug;28(8):833-854
Cell research 2018 Aug;28(8):833-854
Sequence-dependent cargo recognition by SNX-BARs mediates retromer-independent transport of CI-MPR.
Simonetti B, Danson CM, Heesom KJ, Cullen PJ
The Journal of cell biology 2017 Nov 6;216(11):3695-3712
The Journal of cell biology 2017 Nov 6;216(11):3695-3712
Adenovirus Modulates Toll-Like Receptor 4 Signaling by Reprogramming ORP1L-VAP Protein Contacts for Cholesterol Transport from Endosomes to the Endoplasmic Reticulum.
Cianciola NL, Chung S, Manor D, Carlin CR
Journal of virology 2017 Mar 15;91(6)
Journal of virology 2017 Mar 15;91(6)
Neuronal overexpression of human VAPB slows motor impairment and neuromuscular denervation in a mouse model of ALS.
Kim JY, Jang A, Reddy R, Yoon WH, Jankowsky JL
Human molecular genetics 2016 Nov 1;25(21):4661-4673
Human molecular genetics 2016 Nov 1;25(21):4661-4673
The ATF6 pathway of the ER stress response contributes to enhanced viability in glioblastoma.
Dadey DY, Kapoor V, Khudanyan A, Urano F, Kim AH, Thotala D, Hallahan DE
Oncotarget 2016 Jan 12;7(2):2080-92
Oncotarget 2016 Jan 12;7(2):2080-92
The role of endoplasmic reticulum-related BiP/GRP78 in interferon gamma-induced persistent Chlamydia pneumoniae infection.
Shima K, Klinger M, Schütze S, Kaufhold I, Solbach W, Reiling N, Rupp J
Cellular microbiology 2015 Jul;17(7):923-34
Cellular microbiology 2015 Jul;17(7):923-34
Expression of vesicle-associated membrane-protein-associated protein B cleavage products in peripheral blood leukocytes and cerebrospinal fluid of patients with sporadic amyotrophic lateral sclerosis.
Deidda I, Galizzi G, Passantino R, Cascio C, Russo D, Colletti T, La Bella V, Guarneri P
European journal of neurology 2014 Mar;21(3):478-85
European journal of neurology 2014 Mar;21(3):478-85
Widespread aggregation of mutant VAPB associated with ALS does not cause motor neuron degeneration or modulate mutant SOD1 aggregation and toxicity in mice.
Qiu L, Qiao T, Beers M, Tan W, Wang H, Yang B, Xu Z
Molecular neurodegeneration 2013 Jan 3;8:1
Molecular neurodegeneration 2013 Jan 3;8:1
Disease mutations in Rab7 result in unregulated nucleotide exchange and inappropriate activation.
McCray BA, Skordalakes E, Taylor JP
Human molecular genetics 2010 Mar 15;19(6):1033-47
Human molecular genetics 2010 Mar 15;19(6):1033-47
No comments: Submit comment
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 14477-1-AP(VAPB antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using VAPB antibody 14477-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-VAPB (IP:14477-1-AP, 3ug; Detection:14477-1-AP 1:800) with HeLa cells lysate 1840ug.
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human pancreas cancer using 14477-1-AP(VAPB antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human pancreas cancer using 14477-1-AP(VAPB antibody) at dilution of 1:100 (under 40x lens)
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The VAPB antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human VAPB. This antibody recognizes human,mouse,rat antigen. The VAPB antibody has been validated for the following applications: ELISA, WB, IHC, IF, IP, FC analysis.
