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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- ELISA [1]
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- Product number
- H00001159-M04 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00001159-M04, RRID:AB_1145195
- Product name
- Mouse Monoclonal Creatine kinase MT 1B Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. CKMT1B - creatine kinase, mitochondrial 1B (2C8)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Creatine kinase MT 1B Antibody (2C8) [H00001159-M04] - Western blot analysis of CKMT1B over-expressed 293 cell line, cotransfected with CKMT1B Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed H00001159-M04. GAPDH (36.1 kDa) used as loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Creatine kinase MT 1B Antibody (2C8) [H00001159-M04] - Analysis of CKMT1B expression in transfected 293T cell line by CKMT1B monoclonal antibody (M04), clone 2C8. Lane 1: CKMT1B transfected lysatE (47 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Creatine kinase MT 1B Antibody (2C8) [H00001159-M04] - Analysis of CKMT1B expression in A-431 (Cat # L015V1).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Creatine kinase MT 1B Antibody (2C8) [H00001159-M04] - Detection limit for recombinant GST tagged CKMT1B is approximately 0.1ng/ml as a capture antibody.
