H00001646-M03

antibody from Abnova Corporation

Targeting: AKR1C2 BABP, DD, DD2, DDH2, HAKRD, MCDR2, TDD

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00001646-M03

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00001646-M03, RRID:AB_605919

Product name

AKR1C2 monoclonal antibody (M03), clone 3C11

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant AKR1C2.

Antigen sequence

EEPWVDPNSPVLLEDPVLCALAKKHKRTPALIALR
YQLQRGVVVLAKSYNEQRIRQNVQVFEFQLTSEEM
KAIDGLNRNVRYLTLDIFAGPPNYPVSDEY


Isotype

IgG

Antibody clone number

3C11

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Aldo-keto reductases AKR1C1, AKR1C2 and AKR1C3 may enhance progesterone metabolism in ovarian endometriosis.

Hevir N, Vouk K, Sinkovec J, Ribič-Pucelj M, Rižner TL
Chemico-biological interactions 2011 May 30;191(1-3):217-26

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Aldo-keto reductase 1C2 fails to metabolize doxorubicin and daunorubicin in vitro.

Takahashi RH, Bains OS, Pfeifer TA, Grigliatti TA, Reid RE, Riggs KW
Drug metabolism and disposition: the biological fate of chemicals 2008 Jun;36(6):991-4

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of AKR1C2 expression in transfected 293T cell line by AKR1C2 monoclonal antibody (M03), clone 3C11.Lane 1: AKR1C2 transfected lysate(36.7 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged AKR1C2 is approximately 0.3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol