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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PAB3751 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3751, RRID:AB_1570870
- Product name
- ACO2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of ACO2.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Modulation of mitochondrial aconitase on the bioenergy of human prostate carcinoma cells.
Lon protease preferentially degrades oxidized mitochondrial aconitase by an ATP-stimulated mechanism.
The aconitase family: three structural variations on a common theme.
Juang HH
Molecular genetics and metabolism 2004 Mar;81(3):244-52
Molecular genetics and metabolism 2004 Mar;81(3):244-52
Lon protease preferentially degrades oxidized mitochondrial aconitase by an ATP-stimulated mechanism.
Bota DA, Davies KJ
Nature cell biology 2002 Sep;4(9):674-80
Nature cell biology 2002 Sep;4(9):674-80
The aconitase family: three structural variations on a common theme.
Gruer MJ, Artymiuk PJ, Guest JR
Trends in biochemical sciences 1997 Jan;22(1):3-6
Trends in biochemical sciences 1997 Jan;22(1):3-6
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of ACO2 polyclonal antibody (Cat # PAB3751) in mouse heart (left) and 293 (right) cell lysates (35 ug/lane). ACO2 (arrow) was detected using the purified polyclonal antibody (1:1000 dilution).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Perfused isolated rat heart whole tissue lysate was lysed with either A) 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.1% SDS, 0.5% Na-deoxycholate, 1 mM Na3VO4, 20 mM NaF, 1 mM PMSF, 5 v/v % protease inhibitor cocktail or B) T-PER Tissue Protein Extraction Reagent (Pierce), containing 1mM Na3VO4, 20 mM NaF, 5 v/v % protease inhibitor cocktail (Sigma) ; PVDF membrane was incubated in primary Ab ACO2 polyclonal antibody (Cat # PAB3751). Solution : 1 : 1000 diluted in 5% NFM TBS-T 0,05 for overnight (15 hrs) at 4 °C. Data courtesy of Boglarka Laczy M.D., Division of Cardiovascular Disease, Dept. of Medicine, University of Alabama at Birmingham.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human heartreacted with ACO2 polyclonal antibody (Cat # PAB3751), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
