Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 21070002-0.1mg - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal AP3M2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This product is specific for Human AP3M2.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: AP3M2 Antibody [21070002] - Western Blot was performed using affinity purified SEQer AP3M2 antibody, aa(321-418) antibody. The lanes contain 5-30ug of a whole cell extract. Final concentration of antibodies = 0.1ug/ml (1:10,000 dilution). The blot was probed overnight with the SEQer AP3M2 antibody, aa(321-418) antibody. Blot was then washed according to protocol and probed with goat-anti-Rabbit-HRP conjugate at 1:5000 dilution, washed and developed using chemiluminescence (film exposure 5-30sec). The protein was detected as represented by the band shown.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: AP3M2 Antibody [21070002]
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: AP3M2 Antibody [21070002] - Normal tissues showed moderate to strong cytoplasmic staining. Gastro-intestinal glandular cells, hepatocytes and the thyroid exhibited strong staining. The malignant tissues displayed mainly moderate positivity. Strong staining was observed in most cases of colo-rectal cancers but also observed in occasional cases of other malignancies. Skin cancers generally displayed weak staining. Image and statement courtesy of the Human Protein Atlas (HPA).
