Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-21344 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GGT1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Molt-4, Neuro2A, GL261, C8D30, NIH-3T3, Raw264.7, C2C12.
- Concentration
- 0.71 mg/mL
Submitted references Metastatic and non-metastatic sentinel inguinofemoral lymph nodes in vulvar cancer show an increased lymphangiogenesis.
Association of the immunohistochemical detection of gamma-glutamyl transferase expression with clinicopathological findings in postmenopausal women with endometrioid adenocarcinoma of the uterus.
Piechowicz M, Mikos M, Banas T, Okon K, Pietrus M, Balajewicz-Nowak M, Szczudlik L, Kojs Z, Czerw A, Juszczyk G, PityĆski K
Annals of agricultural and environmental medicine : AAEM 2020 Mar 17;27(1):123-128
Annals of agricultural and environmental medicine : AAEM 2020 Mar 17;27(1):123-128
Association of the immunohistochemical detection of gamma-glutamyl transferase expression with clinicopathological findings in postmenopausal women with endometrioid adenocarcinoma of the uterus.
Pitynski K, Ozimek T, Galuszka N, Banas T, Milian-Ciesielska K, Pietrus M, Okon K, Mikos M, Juszczyk G, Sinczak-Kuta A, Stoj A
Journal of physiology and pharmacology : an official journal of the Polish Physiological Society 2016 Jun;67(3):395-402
Journal of physiology and pharmacology : an official journal of the Polish Physiological Society 2016 Jun;67(3):395-402
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GGT1/gamma-Glutamyltransferase 1 using 30 µg of MOLT4 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a GGT1/gamma-Glutamyltransferase 1 polyclonal antibody (Product # PA5-21344) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using GGT1 Polyclonal Antibody (Product # PA5-21344). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GGT1 Polyclonal Antibody (Product # PA5-21344) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GGT1 Polyclonal Antibody detects GGT1 protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract. B. 30 µg GL261 whole cell lysate/extract. C. 30 µg C8D30 whole cell lysate/extract. D. 30 µg NIH-3T3 whole cell lysate/extract. E. 30 µg Raw264.7 whole cell lysate/extract. F. 30 µg C2C12 whole cell lysate/extract.7.5% SDS-PAGE. GGT1 Polyclonal Antibody (Product # PA5-21344) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GGT1 Polyclonal Antibody detects GGT1 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. GGT1 stained by GGT1 Polyclonal Antibody (Product # PA5-21344) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.