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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [19]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [6]
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- Product number
- NB100-65541 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-65541, RRID:AB_964555
- Product name
- Mouse Monoclonal MHC Class II Antibody
- Antibody type
- Monoclonal
- Antigen
- Rat thymocyte membrane glycoproteins
- Description
- For IHC-Paraffin: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose. PLP fixation is recommended for optimal results.
- Reactivity
- Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.25 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Submitted references A Gutsy Move for Cell-Based Regenerative Medicine in Parkinson's Disease: Targeting the Gut Microbiome to Sequester Inflammation and Neurotoxicity.
Gutting the brain of inflammation: A key role of gut microbiome in human umbilical cord blood plasma therapy in Parkinson's disease model.
Beneficial effects of liraglutide (GLP1 analog) in the hippocampal inflammation.
Suppression of islet homeostasis protein thwarts diabetes mellitus progression.
Age-related myelin degradation burdens the clearance function of microglia during aging.
Prenatal lipopolysaccharide does not accelerate progressive dopamine neuron loss in the rat as a result of normal aging.
PECAM-1 engagement counteracts ICAM-1-induced signaling in brain vascular endothelial cells.
Progressive dopamine neuron loss following supra-nigral lipopolysaccharide (LPS) infusion into rats exposed to LPS prenatally.
6-Hydroxydopamine-induced alterations in blood-brain barrier permeability.
Rotenone potentiates dopamine neuron loss in animals exposed to lipopolysaccharide prenatally.
Resting respiratory tract dendritic cells preferentially stimulate T helper cell type 2 (Th2) responses and require obligatory cytokine signals for induction of Th1 immunity.
Resting respiratory tract dendritic cells preferentially stimulate T helper cell type 2 (Th2) responses and require obligatory cytokine signals for induction of Th1 immunity.
Functional activity of CD44 isoforms in haemopoiesis of the rat.
Functional activity of CD44 isoforms in haemopoiesis of the rat.
Preventive effects of early anti-CD4 or anti-CD8 treatment on Borna disease in rats.
CD4 rat x rat and mouse x rat T cell hybridomas produced by fusion of established T cell lines and clones to W/Fu (C58NT)D.
Borna disease virus-induced meningoencephalomyelitis caused by a virus-specific CD4+ T cell-mediated immune reaction.
T-cell lymphoma lines derived from rat thymomas induced by Moloney murine leukemia virus: phenotypic diversity and its implications.
T-cell lymphoma lines derived from rat thymomas induced by Moloney murine leukemia virus: phenotypic diversity and its implications.
Lee JY, Tuazon JP, Corey S, Bonsack B, Acosta S, Ehrhart J, Sanberg PR, Borlongan CV
Stem cell reviews and reports 2019 Oct;15(5):690-702
Stem cell reviews and reports 2019 Oct;15(5):690-702
Gutting the brain of inflammation: A key role of gut microbiome in human umbilical cord blood plasma therapy in Parkinson's disease model.
Lee JY, Tuazon JP, Ehrhart J, Sanberg PR, Borlongan CV
Journal of cellular and molecular medicine 2019 Aug;23(8):5466-5474
Journal of cellular and molecular medicine 2019 Aug;23(8):5466-5474
Beneficial effects of liraglutide (GLP1 analog) in the hippocampal inflammation.
Barreto-Vianna ARC, Aguila MB, Mandarim-de-Lacerda CA
Metabolic brain disease 2017 Oct;32(5):1735-1745
Metabolic brain disease 2017 Oct;32(5):1735-1745
Suppression of islet homeostasis protein thwarts diabetes mellitus progression.
Oh SH, Jorgensen ML, Wasserfall CH, Gjymishka A, Petersen BE
Laboratory investigation; a journal of technical methods and pathology 2017 May;97(5):577-590
Laboratory investigation; a journal of technical methods and pathology 2017 May;97(5):577-590
Age-related myelin degradation burdens the clearance function of microglia during aging.
Safaiyan S, Kannaiyan N, Snaidero N, Brioschi S, Biber K, Yona S, Edinger AL, Jung S, Rossner MJ, Simons M
Nature neuroscience 2016 Aug;19(8):995-8
Nature neuroscience 2016 Aug;19(8):995-8
Prenatal lipopolysaccharide does not accelerate progressive dopamine neuron loss in the rat as a result of normal aging.
Ling Z, Zhu Y, Tong CW, Snyder JA, Lipton JW, Carvey PM
Experimental neurology 2009 Apr;216(2):312-20
Experimental neurology 2009 Apr;216(2):312-20
PECAM-1 engagement counteracts ICAM-1-induced signaling in brain vascular endothelial cells.
Couty JP, Rampon C, Leveque M, Laran-Chich MP, Bourdoulous S, Greenwood J, Couraud PO
Journal of neurochemistry 2007 Oct;103(2):793-801
Journal of neurochemistry 2007 Oct;103(2):793-801
Progressive dopamine neuron loss following supra-nigral lipopolysaccharide (LPS) infusion into rats exposed to LPS prenatally.
Ling Z, Zhu Y, Tong Cw, Snyder JA, Lipton JW, Carvey PM
Experimental neurology 2006 Jun;199(2):499-512
Experimental neurology 2006 Jun;199(2):499-512
6-Hydroxydopamine-induced alterations in blood-brain barrier permeability.
Carvey PM, Zhao CH, Hendey B, Lum H, Trachtenberg J, Desai BS, Snyder J, Zhu YG, Ling ZD
The European journal of neuroscience 2005 Sep;22(5):1158-68
The European journal of neuroscience 2005 Sep;22(5):1158-68
Rotenone potentiates dopamine neuron loss in animals exposed to lipopolysaccharide prenatally.
Ling Z, Chang QA, Tong CW, Leurgans SE, Lipton JW, Carvey PM
Experimental neurology 2004 Dec;190(2):373-83
Experimental neurology 2004 Dec;190(2):373-83
Resting respiratory tract dendritic cells preferentially stimulate T helper cell type 2 (Th2) responses and require obligatory cytokine signals for induction of Th1 immunity.
Stumbles PA, Thomas JA, Pimm CL, Lee PT, Venaille TJ, Proksch S, Holt PG
The Journal of experimental medicine 1998 Dec 7;188(11):2019-31
The Journal of experimental medicine 1998 Dec 7;188(11):2019-31
Resting respiratory tract dendritic cells preferentially stimulate T helper cell type 2 (Th2) responses and require obligatory cytokine signals for induction of Th1 immunity.
Stumbles PA, Thomas JA, Pimm CL, Lee PT, Venaille TJ, Proksch S, Holt PG
The Journal of experimental medicine 1998 Dec 7;188(11):2019-31
The Journal of experimental medicine 1998 Dec 7;188(11):2019-31
Functional activity of CD44 isoforms in haemopoiesis of the rat.
Khaldoyanidi S, Schnabel D, Föhr N, Zöller M
British journal of haematology 1997 Jan;96(1):31-45
British journal of haematology 1997 Jan;96(1):31-45
Functional activity of CD44 isoforms in haemopoiesis of the rat.
Khaldoyanidi S, Schnabel D, Föhr N, Zöller M
British journal of haematology 1997 Jan;96(1):31-45
British journal of haematology 1997 Jan;96(1):31-45
Preventive effects of early anti-CD4 or anti-CD8 treatment on Borna disease in rats.
Stitz L, Sobbe M, Bilzer T
Journal of virology 1992 Jun;66(6):3316-23
Journal of virology 1992 Jun;66(6):3316-23
CD4 rat x rat and mouse x rat T cell hybridomas produced by fusion of established T cell lines and clones to W/Fu (C58NT)D.
Boots AM, van Lierop MJ, Wauben MH, van Kooten PJ, Hensen EJ, van Eden W
Journal of immunological methods 1991 Nov 5;144(1):1-10
Journal of immunological methods 1991 Nov 5;144(1):1-10
Borna disease virus-induced meningoencephalomyelitis caused by a virus-specific CD4+ T cell-mediated immune reaction.
Richt J, Stitz L, Deschl U, Frese K, Rott R
The Journal of general virology 1990 Nov;71 ( Pt 11):2565-73
The Journal of general virology 1990 Nov;71 ( Pt 11):2565-73
T-cell lymphoma lines derived from rat thymomas induced by Moloney murine leukemia virus: phenotypic diversity and its implications.
Lazo PA, Klein-Szanto AJ, Tsichlis PN
Journal of virology 1990 Aug;64(8):3948-59
Journal of virology 1990 Aug;64(8):3948-59
T-cell lymphoma lines derived from rat thymomas induced by Moloney murine leukemia virus: phenotypic diversity and its implications.
Lazo PA, Klein-Szanto AJ, Tsichlis PN
Journal of virology 1990 Aug;64(8):3948-59
Journal of virology 1990 Aug;64(8):3948-59
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MHC Class II RT1B Antibody (OX-6) [NB100-65541] - Rat Spleen Tissue lysate probed with MHC Class II antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MHC Class II RT1B Antibody (OX-6) [NB100-65541] - Total protein from mouse placenta and spleen was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-RT1b in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MHC Class II RT1B Antibody (OX-6) [NB100-65541] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse spleen using MHC Class II RT1B antibody (clone OX-6) at 1:100 dilution. The signal was developed using HRP-labelled secondary antibody and DAB reagent, and the nuclei were counterstained with hematoxylin. The antibody generated very specific signal in a subset of spleenocytes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: MHC Class II RT1B Antibody (OX-6) [NB100-65541] - Staining of rat splenocytes with Mouse anti Rat MHC class II RT1B
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: MHC Class II RT1B Antibody (OX-6) [NB100-65541] - Staining of rat spleen cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: MHC Class II RT1B Antibody (OX-6) [NB100-65541] - Staining of rat spleen cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: MHC Class II RT1B Antibody (OX-6) [NB100-65541] - Staining of rat spleen cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: MHC Class II RT1B Antibody (OX-6) [NB100-65541] - Staining of rat spleen cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: MHC Class II RT1B Antibody (OX-6) [NB100-65541] - Analysis using Alexa Fluor (R) 488 conjugate of NB100-65541. Rat Splenocytes were stained with MHC Class II RT1B (OX-6) antibody NB100-65541 (blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. The antibodies were directly conjugated to Alexa Fluor 488.
