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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- MAB9715 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB9715, RRID:AB_10755013
- Product name
- EIF5A monoclonal antibody, clone AT2E9
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant EIF5A.
- Isotype
- IgG
- Antibody clone number
- AT2E9
- Storage
- Store at 4°C. For long term storage store at -80°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of tissue lysates (35 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with EIF5A monoclonal antibody, clone AT2E9 (Cat # MAB9715) at 1 : 500 dilution. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1 : mouse lung.Lane 2 : mouse kidney.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of human HeLa cells stained with Hoechst 33342 (Blue) for chromatin staining and EIF5A monoclonal antibody, clone AT2E9 (Cat # MAB9715) (1 : 500) dilution with Texas Red (Red).
- Validation comment
- Immunofluorescence
