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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-29204 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- eIF5A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, mouse brain, PC-12, Rat2. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (81%), Xenopus laevis (83%), Sheep (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.4 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Dysregulation of Translation Factors EIF2S1, EIF5A and EIF6 in Intestinal-Type Adenocarcinoma (ITAC).
Schatz C, Sprung S, Schartinger V, Codina-MartÃnez H, Lechner M, Hermsen M, Haybaeck J
Cancers 2021 Nov 11;13(22)
Cancers 2021 Nov 11;13(22)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EIF5A using 50 µg of mouse brain lysate. Samples were loaded onto a 15% SDS-PAGE gel and probed with an EIF5A polyclonal antibody (Product # PA5-29204) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EIF5A using 30 µg of A431 lysate. Samples were loaded onto a 15% SDS-PAGE gel and probed with an EIF5A polyclonal antibody (Product # PA5-29204) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- eIF5A Polyclonal Antibody detects EIF5A protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract. B. 30 µg Rat2 whole cell lysate/extract.12 % SDS-PAGE. EIF5A Polyclonal Antibody (Product # PA5-29204) dilution: 1:5,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using eIF5A Polyclonal Antibody (Product # PA5-29204). Mouse tissue extract (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with eIF5A Polyclonal Antibody (Product # PA5-29204) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of EIF5A in paraformaldehyde-fixed HeLa cells using an EIF5A polyclonal antibody (Product # PA5-29204) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- eIF5A Polyclonal Antibody detects EIF5A protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EIF5A stained by eIF5A Polyclonal Antibody (Product # PA5-29204) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin Polyclonal Antibody [GT114] (Product # MA5-31466) diluted at 1:1,000. Blue: Fluoroshield with DAPI .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of eIF5A was performed in paraffin-embedded human breast carcinoma tissue using eIF5A Polyclonal Antibody (Product # PA5-29204) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 NNT ( upper row ), ITAC ( lower row ). Stained tissue sections using antibodies against EIF2S1, EIF5A and EIF6 in tissue micro arrays (TMAs) at different resolutions.
