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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- GTX83950 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX83950, RRID:AB_10728418
- Product name
- PAICS antibody [2C9]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse
- Host
- Mouse
Submitted references A Role for De Novo Purine Metabolic Enzyme PAICS in Bladder Cancer Progression.
Expression and Role of PAICS, a De Novo Purine Biosynthetic Gene in Prostate Cancer.
Chakravarthi BVSK, Rodriguez Pena MDC, Agarwal S, Chandrashekar DS, Hodigere Balasubramanya SA, Jabboure FJ, Matoso A, Bivalacqua TJ, Rezaei K, Chaux A, Grizzle WE, Sonpavde G, Gordetsky J, Netto GJ, Varambally S
Neoplasia (New York, N.Y.) 2018 Sep;20(9):894-904
Neoplasia (New York, N.Y.) 2018 Sep;20(9):894-904
Expression and Role of PAICS, a De Novo Purine Biosynthetic Gene in Prostate Cancer.
Chakravarthi BV, Goswami MT, Pathi SS, Dodson M, Chandrashekar DS, Agarwal S, Nepal S, Hodigere Balasubramanya SA, Siddiqui J, Lonigro RJ, Chinnaiyan AM, Kunju LP, Palanisamy N, Varambally S
The Prostate 2017 Jan;77(1):10-21
The Prostate 2017 Jan;77(1):10-21
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PAICS monoclonal antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAICS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAICS.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Anti-PAICS mouse monoclonal antibody (GTX83950) immunofluorescent staining of COS7 cells transiently transfected with PAICS
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC215225 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PAICS antibody(GTX83950), and then analyzed by flow cytometry.
