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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- CF501469 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PAICS Monoclonal Antibody (OTI5B6), TrueMAB™
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI5B6
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAICS (RC215225, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-PAICS. Positive lysates LY416636 (100 µg) and LC416636 (20 µg) can be purchased separately from OriGene.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10 µg) from 10 Human tissue by using anti-PAICS monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Anti-PAICS mouse monoclonal antibody (TA501469) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PAICS(RC215225).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Anti-PAICS mouse monoclonal antibody (TA501469) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PAICS(RC215225).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC215225 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PAICS antibody (TA501469), and then analyzed by flow cytometry.
