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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunocytochemistry [3]
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- Product number
- 700076 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RANBP3 Recombinant Rabbit Monoclonal Antibody (B24H15L41)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
- Antibody clone number
- B24H15L41
- Concentration
- 0.5 mg/mL
Submitted references Location-specific inhibition of Akt reveals regulation of mTORC1 activity in the nucleus.
AKT activation drives the nuclear localization of CSE1L and a pro-oncogenic transcriptional activation in ovarian cancer cells.
Zhou X, Zhong Y, Molinar-Inglis O, Kunkel MT, Chen M, Sun T, Zhang J, Shyy JY, Trejo J, Newton AC, Zhang J
Nature communications 2020 Nov 30;11(1):6088
Nature communications 2020 Nov 30;11(1):6088
AKT activation drives the nuclear localization of CSE1L and a pro-oncogenic transcriptional activation in ovarian cancer cells.
Lorenzato A, Biolatti M, Delogu G, Capobianco G, Farace C, Dessole S, Cossu A, Tanda F, Madeddu R, Olivero M, Di Renzo MF
Experimental cell research 2013 Oct 15;319(17):2627-36
Experimental cell research 2013 Oct 15;319(17):2627-36
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RANBP3 in Jurkat cell lysate (30 µg/lane) using a RANBP3 recombinant rabbit monoclonal antibody (Product # 700076) at a dilution of 0.5 µg/mL. NBT/BCIP was used as the substrate (Product # WB7105).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RANBP3 in Jurkat cell lysate (30 µg/lane) using a RANBP3 recombinant rabbit monoclonal antibody (Product # 700076) at a dilution of 0.5 µg/mL. NBT/BCIP was used as the substrate (Product # WB7105).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of RANBP3 in HeLa cells using a RANBP3 recombinant rabbit monoclonal antibody (Product # 700076) at a dilution of 5 µg/mL followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody at a dilution of 1:1000. Cells were counterstained with Alexa Fluor 568 Phalliodin (Product # A12380) and treated with SlowFade Gold with DAPI (Product # S36936).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of RANBP3 Antibody (B24H15L41), Recombinant Rabbit Monoclonal Antibody was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with RANBP3 (B24H15L41) Recombinant Rabbit Monoclonal Antibody (Product # 700076) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with 4 µg/mL Donkey Anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 546 (Product # A10040) for 45 minutes at room temperature (Panel a: red). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: green) was stained with Alexa Fluor® 488 Phalloidin (Product # A12379, 1:300). Panel d represents the merged image showing nuclear localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of RANBP3 in HeLa cells using a RANBP3 recombinant rabbit monoclonal antibody (Product # 700076) at a dilution of 5 µg/mL followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody at a dilution of 1:1000. Cells were counterstained with Alexa Fluor 568 Phalliodin (Product # A12380) and treated with SlowFade Gold with DAPI (Product # S36936).
