H00008498-M01

antibody from Abnova Corporation

Targeting: RANBP3

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00008498-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00008498-M01, RRID:AB_464355

Product name

RANBP3 monoclonal antibody (M01), clone 2E10

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant RANBP3.

Antigen sequence

KESLAESAAAYTKATARKCLLEKVEVITGEEAESN
VLQMQCKLFVFDKTSQSWLLRHPHPSHPAVGPCLC
GEQPALCPCPGLPNYRPGTPAQLGGLLVRV


Isotype

IgG

Antibody clone number

2E10

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of RANBP3 expression in transfected 293T cell line by RANBP3 monoclonal antibody (M01), clone 2E10.Lane 1: RANBP3 transfected lysate(42.4 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged RANBP3 is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to RANBP3 on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol