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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- SM6029 - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#SM6029, RRID:AB_972541
- Product name
- anti Adenylate kinase 3 (AK3)
- Antibody type
- Monoclonal
- Antigen
- Recombinant human AK3 (adenylaste kinase isozyme 3) purified from E. coli
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- SJB3-36
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis: The recombinant human AK isozymes (AK1(1), AK2(2) and AK3(3)) and mouse liver(4) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-AK3 antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry: Human liver tissue was incubated with anti-human AK3 (1:100) for 2 hours at room temperature. Slide was then washed in PBS, and was incubated in avidin biosystem anti-rabbit labeled polymer for 30 min at RT. Enzyme detection was performed with DAB chromogen.
