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LearnAF6957
antibody from R&D Systems
Targeting: AKR1C4
3-alpha-HSD, C11, CDR, CHDR, DD4, HAKRA, MGC22581
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
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- Product number
- AF6957 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Aldo-keto Reductase 1C4/AKR1C4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Aldo-keto Reductase 1C4/AKR1C4 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) AKR-1C1 and rhAKR-1C3 is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
NP_001809- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Aldo-keto Reductase 1C4/AKR1C4 by Western Blot. Western blot shows lysates of human liver tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Aldo-keto Reductase 1C4/AKR1C4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6957) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Aldo-keto Reductase 1C4/AKR1C4 at approximately 35-36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
