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LearnH00001109-M01
antibody from Abnova Corporation
Targeting: AKR1C4
3-alpha-HSD, C11, CDR, CHDR, DD4, HAKRA, MGC22581
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00001109-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00001109-M01, RRID:AB_425366
- Product name
- AKR1C4 monoclonal antibody (M01), clone 2C11
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant AKR1C4.
- Antigen sequence
MDPKYQRVELNDGHFMPVLGFGTYAPPEVPRNRAV
EVTKLAIEAGFRHIDSAYLYNNEEQVGLAIRSKIA
DGSVKREDIFYTSKLWCTFFQPQMVQPALESSLKK
LQLDYVDLYLLHFPMALKPGETPLPKDENGKVIFD
TVDLSATWEVMEKCKDAGLAKSIGVSNFNYRQLEM
ILNKPGLKYKPVCNQVECHPYLNQSKLLDFCKSKD
IVLVAHSALGTQRHKLWVDPNSPVLLEDPVLCALA
KKHKRTPALIALRYQLQRGVVVLAKSYNEQRIREN
IQVFEFQLTSEDMKVLDGLNRNYRYVVMDFLMDHP
DYPFSDEY- Isotype
- IgG
- Antibody clone number
- 2C11
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Regulation of human 3 alpha-hydroxysteroid dehydrogenase (AKR1C4) expression by the liver X receptor alpha.
Stayrook KR, Rogers PM, Savkur RS, Wang Y, Su C, Varga G, Bu X, Wei T, Nagpal S, Liu XS, Burris TP
Molecular pharmacology 2008 Feb;73(2):607-12
Molecular pharmacology 2008 Feb;73(2):607-12
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- AKR1C4 monoclonal antibody (M01), clone 2C11 Western Blot analysis of AKR1C4 expression in MCF-7 ( Cat # L046V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of AKR1C4 expression in transfected 293T cell line by AKR1C4 monoclonal antibody (M01), clone 2C11.Lane 1: AKR1C4 transfected lysate(37.2 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged AKR1C4 is 3 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to AKR1C4 on HepG2 cell. [antibody concentration 15 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to AKR1C4 on formalin-fixed paraffin-embedded human liver tissue. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
