H00001109-M01
antibody from Novus Biologicals
Targeting: AKR1C4
3-alpha-HSD, C11, CDR, CHDR, DD4, HAKRA, MGC22581
Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00001109-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00001109-M01, RRID:AB_536686
- Product name
- Mouse Monoclonal Aldo-keto Reductase 1C4/AKR1C4 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. AKR1C4 - aldo-keto reductase family 1, member C4 (chlordecone reductase
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Modulation of cell metabolic pathways and oxidative stress signaling contribute to acquired melphalan resistance in multiple myeloma cells.
Regulation of human 3 alpha-hydroxysteroid dehydrogenase (AKR1C4) expression by the liver X receptor alpha.
Zub KA, Sousa MM, Sarno A, Sharma A, Demirovic A, Rao S, Young C, Aas PA, Ericsson I, Sundan A, Jensen ON, Slupphaug G
PloS one 2015;10(3):e0119857
PloS one 2015;10(3):e0119857
Regulation of human 3 alpha-hydroxysteroid dehydrogenase (AKR1C4) expression by the liver X receptor alpha.
Stayrook KR, Rogers PM, Savkur RS, Wang Y, Su C, Varga G, Bu X, Wei T, Nagpal S, Liu XS, Burris TP
Molecular pharmacology 2008 Feb;73(2):607-12
Molecular pharmacology 2008 Feb;73(2):607-12
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Aldo-keto Reductase 1C4/AKR1C4 Antibody (2C11) [H00001109-M01] - Analysis of AKR1C4 expression in transfected 293T cell line by AKR1C4 monoclonal antibody (M01), clone 2C11.Lane 1: AKR1C4 transfected lysate(37.2 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Aldo-keto Reductase 1C4/AKR1C4 Antibody (2C11) [H00001109-M01] - AKR1C4 monoclonal antibody (M01), clone 2C11 Analysis of AKR1C4 expression in MCF-7.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: Aldo-keto Reductase 1C4/AKR1C4 Antibody (2C11) [H00001109-M01] - Detection limit for recombinant GST tagged AKR1C4 is 3 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Aldo-keto Reductase 1C4/AKR1C4 Antibody (2C11) [H00001109-M01] - Analysis of monoclonal antibody to AKR1C4 on HepG2 cell. Antibody concentration 15 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Aldo-keto Reductase 1C4/AKR1C4 Antibody (2C11) [H00001109-M01] - Analysis of monoclonal antibody to AKR1C4 on formalin-fixed paraffin-embedded human liver tissue. Antibody concentration 3 ug/ml.