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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [2]
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- Product number
- 702434 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- JAK2 Recombinant Rabbit Monoclonal Antibody (18H11L8)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with Monkey, Rat, Sheep and Bovine.
- Antibody clone number
- 18H11L8
- Concentration
- 0.5 mg/mL
Submitted references Discovery of a novel potentially transforming somatic mutation in CSF2RB gene in breast cancer.
Isolation and Establishment of a Highly Proliferative, Cancer Stem Cell-Like, and Naturally Immortalized Triple-Negative Breast Cancer Cell Line, KAIMRC2.
Role and mechanism of nursing cooperation and tetramethylpyrazine application in post-operative pain in patients undergoing total knee arthroplasty.
Rashid M, Ali R, Almuzzaini B, Song H, AlHallaj A, Abdulkarim AA, Mohamed Baz O, Al Zahrani H, Mustafa Sabeena M, Alharbi W, Hussein M, Boudjelal M
Cancer medicine 2021 Nov;10(22):8138-8150
Cancer medicine 2021 Nov;10(22):8138-8150
Isolation and Establishment of a Highly Proliferative, Cancer Stem Cell-Like, and Naturally Immortalized Triple-Negative Breast Cancer Cell Line, KAIMRC2.
Ali R, Al Zahrani H, Barhoumi T, Alhallaj A, Mashhour A, Alshammari MA, Alshawakir YA, Baz O, Alanazi AH, Khan AL, Al Nikhli H, Al Balwi MA, Al Riyees L, Boudjelal M
Cells 2021 May 24;10(6)
Cells 2021 May 24;10(6)
Role and mechanism of nursing cooperation and tetramethylpyrazine application in post-operative pain in patients undergoing total knee arthroplasty.
Liu C, Liu R, Tang M, Yang X, Gong X
Experimental and therapeutic medicine 2019 Mar;17(3):2366-2372
Experimental and therapeutic medicine 2019 Mar;17(3):2366-2372
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of HT-29 (Lane 1), HT-29 treated with 20 uM MG132 for 1 hour and then with 100 ng/mL IFN-gamma for 15 mins (Lane 2), HEL (Lane 3) and HEL treated with 20 uM MG132 for 1 hour and then with 100 ng/mL IFN-gamma for 15 mins (Lane 4). The blots were probed with Jak2 Recombinant Rabbit Monoclonal Antibody (Product # 702434, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 131 kDa band corresponding to Jak2 with the total levels of the protein increasing upon corresponding treatment was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- For immunofluorescence analysis, MG-132 (20 uM, 1 h) and IFN gamma (100 ng, 15 min) treated HT-29 cells were fixed and permeabilized for detection of endogenous Jak2 using Anti-Jak2 Recombinant Rabbit Monoclonal Antibody (Product # 702434, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of Jak2 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating membrane localization of Jak2. Panel e) shows untreated cells with no signal. Panel f) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 2 FIGURE Characterization of KAIMRC1 cells on protein level. (A) Western blot analysis of the KAIMRC1 cell line in normal (+) and serum-starved conditions(-) against P- CSF2RB , CSF2RB , P-AKT1, AKT1, JAK2, P-JAK2, STAT3, P-STAT3, STAT5, mTOR, and P-mTOR. Ligand-independent activation of AKT/mTOR pathway, as well as the JAK2/STAT pathway, was observed. (B) Comparative Western blot analysis of KAIMRC1, MDA-MB-231, and MCF-7 cell lines against CSF2RB . (C) Schematic representation of proposed activation of the AKT/mTOR pathway and JAK2/STAT pathway that might result in cell cycle survival and proliferation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 2 Characterization of KAIMRC1 cells on protein level. (A) Western blot analysis of the KAIMRC1 cell line in normal (+) and serum-starved conditions(-) against P- CSF2RB , CSF2RB , P-AKT1, AKT1, JAK2, P-JAK2, STAT3, P-STAT3, STAT5, mTOR, and P-mTOR. Ligand-independent activation of AKT/mTOR pathway, as well as the JAK2/STAT pathway, was observed. (B) Comparative Western blot analysis of KAIMRC1, MDA-MB-231, and MCF-7 cell lines against CSF2RB . (C) Schematic representation of proposed activation of the AKT/mTOR pathway and JAK2/STAT pathway that might result in cell cycle survival and proliferation
