22015-1-AP

antibody from Invitrogen Antibodies

Targeting: RRBP1 ES/130, hES

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

22015-1-AP

Provider

Invitrogen Antibodies

Product name

RRBP1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: AQTMEALLA LLPELSVLAQ QNYTEWLQDL KEKGPTLLKH PPAPAEPSSD LASKLREAEE TQSTLQAECD QYRSILAETE GMLRDLQKSV EEEEQVWRAK VGAAEEELQK SRVTVKHLEE IVEKLKGELE SSDQVREHTL HLEAELEKHM AAASAECQNY AKEVAGLRQL LLESQSQLDA AKSEAQKQSD ELALVRQQLS EMKSHVEDGD IAGAPASSPE APPAEQDPVQ LKTQLEWTEA ILEDEQTQRQ KLTAEFEEAQ TSACRLQEEL EKLRTAGPLE SSETEEASQL KERLEKEKKL TSDLGRAATR LQELLKTTQE QLAREKDTVK KLQEQLEKAE DGSSSKEGTS V (1061-1410 aa encoded by BC128577)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.67 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 22015-1-AP (RRBP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22015-1-AP (RRBP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of NIH/3T3 cells using 22015-1-AP (RRBP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of NIH/3T3 cells using 22015-1-AP (RRBP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas tissue slide using 22015-1-AP (RRBP1 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas tissue slide using 22015-1-AP (RRBP1 Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 22015-1-AP ( RRBP1 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 22015-1-AP ( RRBP1 Antibody) at dilution of 1:50 (under 40x lens).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 BxPC-3 cells were stained with 0.2ug RRBP1 antibody (22015-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.