21009-1-AP

antibody from Invitrogen Antibodies

Targeting: CRYBB3 CRYB3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

21009-1-AP

Provider

Invitrogen Antibodies

Product name

CRYBB3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: QGKRCELSA ECPSLTDSLL EKVGSIQVES GPWLAFESRA FRGEQFVLEK GDYPRWDAWS NSRDSDSLLS LRPLNIDSPH HKLHLFENPA FSGRKMEIVD DDVPSLWAHG FQDRVASVRA INGTW (35-158 aa encoded by B C069479)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.49 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse eye tissue were subjected to SDS PAGE followed by western blot with 21009-1-AP (CRYBB3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using CRYBB3 antibody 21009-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using CRYBB3 antibody 21009-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human stomach tissue slide using 21009-1-AP ( CRYBB3 Antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human stomach tissue slide using 21009-1-AP ( CRYBB3 Antibody) at dilution of 1:200 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using (Product # 21009-1-AP) (CRYBB3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).