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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- 21138-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KIAA0528 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: RSRLLRSQS ESSDEVTELD LSHGKKDAFV LEIDDTDAME DVHSLLTDVP PPSGFYSCNT EIMPGINNWT SEIQMFTSVR VIRLSSLNLT NQALNKNFND LCENLLKSLY FKLRSMIPCC LCHVNFTVSL PEDELIQVTV TAVAITFDKN QALQTTKTPV EKSLQRASTD NEELLQFPLE LCSDSLPSHP FPPAKAMTVE KASPVGDGNF RNRSAPPCAN STVGVVKMTP LSFIPGAKIT KYLGIINMFF IRETTSLREE GGVSGFLHAF IAEVFAMVRA HVAALGGNAV VSYIMKQCVF MENPNKNQAQ CLINVSGDAV VFVRESDLEV VSSQQPTTNC QSSCTEGEVT T (651-1000 aa encoded by BC042498)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.28 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 21138-1-AP (KIAA0528 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 21138-1-AP (KIAA0528 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using KIAA0528 antibody 21138-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-KIAA0528 (IP:21138-1-AP, 4ug; Detection:21138-1-AP 1:700) with HeLa cells lysate 520ug.
