TA507087

antibody from OriGene

Targeting: CD274 B7-H, B7-H1, B7H1, PD-L1, PDCD1LG1, PDL1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

TA507087

Provider

OriGene

Proper citation

OriGene Cat#TA507087, RRID:AB_2624079

Product name

PD-L1 / CD274 mouse monoclonal antibody, clone OTI2C7 (formerly 2C7)

Antibody type

Monoclonal

Description

PD-L1 / CD274 mouse monoclonal antibody, clone OTI2C7 (formerly 2C7)

Host

Mouse

Conjugate

Unconjugated

Epitope

CD274

Isotype

IgG

Antibody clone number

OTI2C7

Vial size

100 µl

Concentration

1.00mg/ml

References

Submitted references

Clinicopathological analysis of epithelioid inflammatory myofibroblastic sarcoma.

Du X, Gao Y, Zhao H, Li B, Xue W, Wang D
Oncology letters 2018 Jun;15(6):9317-9326

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD274 (RC213071, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD274.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-CD274 mouse monoclonal antibody (TA507087) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD274(RC213071).

Validation comment

IF

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunocytochemistry staining of CD274 stable expression cells using anti-CD274 mouse monoclonal antibody (TA507087)(Left). The right is negative control.(1:5000)

Validation comment

IF