ARG55930

antibody from Arigo

Targeting: CD274 B7-H, B7-H1, B7H1, PD-L1, PDCD1LG1, PDL1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

ARG55930

Provider

Arigo

Product name

anti-CD274 / PD-L1 antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide around 17 aa (Center) of Human CD274 / PD-L1

Description

Affinity purification with immunogen.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

50 µg

Concentration

1 mg/ml

Storage

For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.

Handling

The antibody solution should be gently mixed before use.

Western blot

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Western blot: Raji cell lysate stained with ARG55930 anti-CD274 / PD-L1 antibody at 1) 0.5 and 2) 1 µg/ml dilution.

Immunocytochemistry

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Immunofluorescence: Human Heart cells stained with ARG55930 anti-CD274 / PD-L1 antibody at 20 µg/ml dilution.

Immunohistochemistry

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Immunohistochemistry: Human tonsil tissue stained with ARG55930 anti-CD274 / PD-L1 antibody at 5 µg/ml.

Flow cytometry

Supportive validation

Submitted by

Arigo (provider)

Main image

Experimental details

Flow Cytometry: A-20 cells stained with ARG55930 anti-CD274 / PD-L1 antibody at 0.5 µg/ml dilution (red); Isotype control (green).