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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB6732 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB6732, RRID:AB_1572316
- Product name
- CD274 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of CD274.
- Antigen sequence
CKKQSDTHLEET- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Costimulatory B7-H1 in renal cell carcinoma patients: Indicator of tumor aggressiveness and potential therapeutic target.
Thompson RH, Gillett MD, Cheville JC, Lohse CM, Dong H, Webster WS, Krejci KG, Lobo JR, Sengupta S, Chen L, Zincke H, Blute ML, Strome SE, Leibovich BC, Kwon ED
Proceedings of the National Academy of Sciences of the United States of America 2004 Dec 7;101(49):17174-9
Proceedings of the National Academy of Sciences of the United States of America 2004 Dec 7;101(49):17174-9
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- CD274 polyclonal antibody (Cat # PAB6732) staining (0.3 ug/mL) of human placenta lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- CD274 polyclonal antibody (Cat # PAB6732) (4 ug/mL) staining of paraffin embedded human placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
