12875-1-AP

antibody from Invitrogen Antibodies

Targeting: PPEF1 PPEF, PPP7CA

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

12875-1-AP

Provider

Invitrogen Antibodies

Product name

PPEF1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Aliquoting is unnecessary for -20°C storage.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

267 µg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 12875-1-AP (PPEF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WB result of PPEF1 antibody (12875-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPEF1 transfected HeLa cells.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12875-1-AP (PPEF1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12875-1-AP (PPEF1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).