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LearnMAB165-100
antibody from R&D Systems
Targeting: ICOSLG
B7-H2, B7h, B7H2, B7RP-1, B7RP1, CD275, GL50, ICOS-L, ICOSL, KIAA0653
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [1]
- Blocking/Neutralizing [1]
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- Product number
- MAB165-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human B7-H2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human B7-H2 in direct ELISAs. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) B7-1, rhB7-2, rhB7-H1, rhB7-H3, or recombinant mouse B7-H2 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
O75144- Isotype
- IgG
- Antibody clone number
- 136726
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references ICOS-LICOS interaction is critically involved in TGN1412-mediated T-cell activation.
ICOS ligation recruits the p50alpha PI3K regulatory subunit to the immunological synapse.
Weissmüller S, Semmler LY, Kalinke U, Christians S, Müller-Berghaus J, Waibler Z
Blood 2012 Jun 28;119(26):6268-77
Blood 2012 Jun 28;119(26):6268-77
ICOS ligation recruits the p50alpha PI3K regulatory subunit to the immunological synapse.
Fos C, Salles A, Lang V, Carrette F, Audebert S, Pastor S, Ghiotto M, Olive D, Bismuth G, Nunès JA
Journal of immunology (Baltimore, Md. : 1950) 2008 Aug 1;181(3):1969-77
Journal of immunology (Baltimore, Md. : 1950) 2008 Aug 1;181(3):1969-77
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of B7-H2 in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human B7-H2 Monoclonal Antibody (Catalog # MAB165, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by B7-H2 and Neutralization by Human B7-H2 Antibody. In the presence of Human CD3 epsilon Monoclonal Antibody (20 ng/mL, Catalog # MAB100), Recombinant Human B7-H2 Fc Chimera (Catalog # 165-B7) stimulates proliferation in PHA-activated human T cells in a dose-dependent manner (orange line). Under these conditions, proliferation elicited by Recombinant Human B7-H2 Fc Chimera (3 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human B7-H2 Monoclonal Antibody (Catalog # MAB165). The ND50 is typically 1-4 µg/mL.
