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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- TA500382 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500382, RRID:AB_2187295
- Product name
- SIGLEC9 mouse monoclonal antibody, clone OTI6D8 (formerly 6D8)
- Antibody type
- Monoclonal
- Description
- SIGLEC9 mouse monoclonal antibody, clone OTI6D8 (formerly 6D8)
- Reactivity
- Canine
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- SIGLEC9
- Isotype
- IgG
- Antibody clone number
- OTI6D8
- Vial size
- 100 µl
- Concentration
- 0.6 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SIGLEC9 monoclonal antibody.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIGLEC9 (RC206674, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIGLEC9.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HT29 cells using anti-SIGLEC9 mouse monoclonal antibody (TA500382).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-SIGLEC9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500382)
- Validation comment
- IHC
