703464

antibody from Invitrogen Antibodies

Targeting: SIGLEC9 CD329

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

703464

Provider

Invitrogen Antibodies

Product name

SIGLEC9 Recombinant Rabbit Monoclonal Antibody (8H11L21)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with Mouse, Rat, Bovine.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

8H11L21

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Moraxella catarrhalis evades neutrophil oxidative stress responses providing a safer niche for nontypeable Haemophilus influenzae.

Nicchi S, Giusti F, Carello S, Utrio Lanfaloni S, Tavarini S, Frigimelica E, Ferlenghi I, Rossi Paccani S, Merola M, Delany I, Scarlato V, Maione D, Brettoni C
iScience 2022 Mar 18;25(3):103931

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-SIGLEC9 Recombinant Rabbit Monoclonal Antibody (Product # 703464) and two bands at 52, 50 kDa corresponding to isoforms of SIGLEC9 was observed in the cell line tested. Membrane enriched extracts (30 µg lysate) of THP-1 (Lane 1) and THP-1 differentiated to M1 Macrophages (Lane 2) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:5000 dilution) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-SIGLEC9 Recombinant Rabbit Monoclonal Antibody (Product # 703464) and two bands at 52, 50 kDa corresponding to isoforms of SIGLEC9 was observed in the cell line tested. Membrane enriched extracts (30 µg lysate) of THP-1 (Lane 1) and THP-1 differentiated to M2 Macrophages (Lane 2) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:5000 dilution) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SIGLEC9 was immunoprecipitated using 5 µg of the Anti-SIGLEC9 Recombinant Rabbit Monoclonal Antibody (Product # 703464) from whole cell extracts (200µg) of THP-1 (Lane 4) using the Dynabeads® Protein G Immunoprecipitation Kit (Product # 10007D). Normal Rabbit IgG was used as a Isotype control (Lane 3). Western blot analysis was performed using Anti-SIGLEC9 Recombinant Rabbit Monoclonal Antibody (Product # 703464, 1:500 dilution). A 55, 60 kDa band corresponding to isoforms of SIGLEC9 was observed in IP elute. The blot was detected by chemiluminescence using Peroxidase IgG Fraction Monoclonal Mouse Anti-Rabbit IgG, light chain specific antibody (Product # 211-032-171, 1:20000 dilution). Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10% Bis-Tris gel (Product # NP03011BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SIGLEC9 was immunoprecipitated using 5 µg of the Anti-SIGLEC9 Recombinant Rabbit Monoclonal Antibody (Product # 703464) from whole cell extracts (200µg) of THP-1 (Lane 4) using the Dynabeads® Protein G Immunoprecipitation Kit (Product # 10007D). Normal Rabbit IgG was used as a Isotype control (Lane 3). Western blot analysis was performed using Anti-SIGLEC9 Recombinant Rabbit Monoclonal Antibody (Product # 703464, 1:500 dilution). A 55, 60 kDa band corresponding to isoforms of SIGLEC9 was observed in IP elute. The blot was detected by chemiluminescence using Peroxidase IgG Fraction Monoclonal Mouse Anti-Rabbit IgG, light chain specific antibody (Product # 211-032-171, 1:20000 dilution). Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10% Bis-Tris gel (Product # NP03011BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).