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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- BAF1139 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Siglec-9 Biotinylated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Siglec-9 in direct ELISAs and Western blots. In direct ELISAs, less than 0.2% cross-reactivity with recombinant human (rh) Siglec-2, rhSiglec-3, rhSiglec-5, rhSiglec-7, and rhSiglec-10 is observed. In Western blots, approximately 25% cross-reactivity with rhSiglec-7 is observed, approximately 5% cross-reactivity with rhSiglec-3 and rhSiglec-5 is observed, and less than 1% cross-reactivity with rhSiglec-2 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Biotin
- Antigen sequence
Q9Y336- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Siglecs facilitate HIV-1 infection of macrophages through adhesion with viral sialic acids.
Zou Z, Chastain A, Moir S, Ford J, Trandem K, Martinelli E, Cicala C, Crocker P, Arthos J, Sun PD
PloS one 2011;6(9):e24559
PloS one 2011;6(9):e24559
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Siglec-9 in Human PBMCs. Siglec-9 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human Siglec-9 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1139) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Siglec-9 in Human Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Goat Anti-Human Siglec-9 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1139, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).
