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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PAB2836 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2836, RRID:AB_1574626
- Product name
- EIF4A1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of EIF4A1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references PDCD4 inhibits translation initiation by binding to eIF4A using both its MA3 domains.
Anti-inflammatory lipid mediator 15d-PGJ2 inhibits translation through inactivation of eIF4A.
Suzuki C, Garces RG, Edmonds KA, Hiller S, Hyberts SG, Marintchev A, Wagner G
Proceedings of the National Academy of Sciences of the United States of America 2008 Mar 4;105(9):3274-9
Proceedings of the National Academy of Sciences of the United States of America 2008 Mar 4;105(9):3274-9
Anti-inflammatory lipid mediator 15d-PGJ2 inhibits translation through inactivation of eIF4A.
Kim WJ, Kim JH, Jang SK
The EMBO journal 2007 Dec 12;26(24):5020-32
The EMBO journal 2007 Dec 12;26(24):5020-32
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of EIF4A1 polyclonal antibody (Cat # PAB2836) in K-562 cell line lysates (35 ug/lane). EIF4A1 (arrow) was detected using the purified polyclonal antibody.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of EIF4A1 (arrow) using rabbit EIF4A1 polyclonal antibody (Cat # PAB2836). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EIF4A1 gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with EIF4A1 polyclonal antibody (Cat # PAB2836) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
