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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- H00055288-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00055288-M01, RRID:AB_2179528
- Product name
- Mouse Monoclonal Rhot1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. RHOT1 - ras homolog gene family, member T1
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Mitofusin 2 Regulates Axonal Transport of Calpastatin to Prevent Neuromuscular Synaptic Elimination in Skeletal Muscles.
Parkin is a disease modifier in the mutant SOD1 mouse model of ALS.
Hypertonia-linked protein Trak1 functions with mitofusins to promote mitochondrial tethering and fusion.
Mitochondrial targeted HSP90 inhibitor Gamitrinib-TPP (G-TPP) induces PINK1/Parkin-dependent mitophagy.
Brain-derived neurotrophic factor (BDNF)-induced mitochondrial motility arrest and presynaptic docking contribute to BDNF-enhanced synaptic transmission.
GTPase of the immune-associated nucleotide-binding protein 5 (GIMAP5) regulates calcium influx in T-lymphocytes by promoting mitochondrial calcium accumulation.
N-acetylglucosamine transferase is an integral component of a kinesin-directed mitochondrial trafficking complex.
GTPase dependent recruitment of Grif-1 by Miro1 regulates mitochondrial trafficking in hippocampal neurons.
Wang L, Gao J, Liu J, Siedlak SL, Torres S, Fujioka H, Huntley ML, Jiang Y, Ji H, Yan T, Harland M, Termsarasab P, Zeng S, Jiang Z, Liang J, Perry G, Hoppel C, Zhang C, Li H, Wang X
Cell metabolism 2018 Sep 4;28(3):400-414.e8
Cell metabolism 2018 Sep 4;28(3):400-414.e8
Parkin is a disease modifier in the mutant SOD1 mouse model of ALS.
Palomo GM, Granatiero V, Kawamata H, Konrad C, Kim M, Arreguin AJ, Zhao D, Milner TA, Manfredi G
EMBO molecular medicine 2018 Oct;10(10)
EMBO molecular medicine 2018 Oct;10(10)
Hypertonia-linked protein Trak1 functions with mitofusins to promote mitochondrial tethering and fusion.
Lee CA, Chin LS, Li L
Protein & cell 2018 Aug;9(8):693-716
Protein & cell 2018 Aug;9(8):693-716
Mitochondrial targeted HSP90 inhibitor Gamitrinib-TPP (G-TPP) induces PINK1/Parkin-dependent mitophagy.
Fiesel FC, James ED, Hudec R, Springer W
Oncotarget 2017 Dec 5;8(63):106233-106248
Oncotarget 2017 Dec 5;8(63):106233-106248
Brain-derived neurotrophic factor (BDNF)-induced mitochondrial motility arrest and presynaptic docking contribute to BDNF-enhanced synaptic transmission.
Su B, Ji YS, Sun XL, Liu XH, Chen ZY
The Journal of biological chemistry 2014 Jan 17;289(3):1213-26
The Journal of biological chemistry 2014 Jan 17;289(3):1213-26
GTPase of the immune-associated nucleotide-binding protein 5 (GIMAP5) regulates calcium influx in T-lymphocytes by promoting mitochondrial calcium accumulation.
Chen XL, Serrano D, Mayhue M, Wieden HJ, Stankova J, Boulay G, Ilangumaran S, Ramanathan S
The Biochemical journal 2013 Jan 15;449(2):353-64
The Biochemical journal 2013 Jan 15;449(2):353-64
N-acetylglucosamine transferase is an integral component of a kinesin-directed mitochondrial trafficking complex.
Brickley K, Pozo K, Stephenson FA
Biochimica et biophysica acta 2011 Jan;1813(1):269-81
Biochimica et biophysica acta 2011 Jan;1813(1):269-81
GTPase dependent recruitment of Grif-1 by Miro1 regulates mitochondrial trafficking in hippocampal neurons.
MacAskill AF, Brickley K, Stephenson FA, Kittler JT
Molecular and cellular neurosciences 2009 Mar;40(3):301-12
Molecular and cellular neurosciences 2009 Mar;40(3):301-12
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Rhot1 Antibody (4H4) [H00055288-M01] - RHOT1 monoclonal antibody (M01), clone 4H4 Analysis of RHOT1 expression in Hela S3 NE.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Rhot1 Antibody (4H4) [H00055288-M01] - Detection limit for recombinant GST tagged RHOT1 is approximately 0.3ng/ml as a capture antibody.
