NBP1-89011

antibody from Novus Biologicals

Targeting: RHOT1 ARHT1, FLJ11040, MIRO-1, MIRO1

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-89011

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-89011, RRID:AB_11034143

Product name

Rabbit Polyclonal Rhot1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human Rhot1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Miro1 Regulates Activity-Driven Positioning of Mitochondria within Astrocytic Processes Apposed to Synapses to Regulate Intracellular Calcium Signaling.

Stephen TL, Higgs NF, Sheehan DF, Al Awabdh S, López-Doménech G, Arancibia-Carcamo IL, Kittler JT
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Dec 2;35(48):15996-6011

---

Mitotic redistribution of the mitochondrial network by Miro and Cenp-F.

Kanfer G, Courthéoux T, Peterka M, Meier S, Soste M, Melnik A, Reis K, Aspenström P, Peter M, Picotti P, Kornmann B
Nature communications 2015 Aug 11;6:8015

---

Lysine 27 ubiquitination of the mitochondrial transport protein Miro is dependent on serine 65 of the Parkin ubiquitin ligase.

Birsa N, Norkett R, Wauer T, Mevissen TE, Wu HC, Foltynie T, Bhatia K, Hirst WD, Komander D, Plun-Favreau H, Kittler JT
The Journal of biological chemistry 2014 May 23;289(21):14569-82

---

The mitochondrial deubiquitinase USP30 opposes parkin-mediated mitophagy.

Bingol B, Tea JS, Phu L, Reichelt M, Bakalarski CE, Song Q, Foreman O, Kirkpatrick DS, Sheng M
Nature 2014 Jun 19;510(7505):370-5

---

Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.

Stadler C, Rexhepaj E, Singan VR, Murphy RF, Pepperkok R, Uhlén M, Simpson JC, Lundberg E
Nature methods 2013 Apr;10(4):315-23

---

VAPB interacts with the mitochondrial protein PTPIP51 to regulate calcium homeostasis.

De Vos KJ, Mórotz GM, Stoica R, Tudor EL, Lau KF, Ackerley S, Warley A, Shaw CE, Miller CC
Human molecular genetics 2012 Mar 15;21(6):1299-311

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Rhot1 Antibody [NBP1-89011] - Staining of human cerebellum shows positivity in neuronal cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Rhot1 Antibody [NBP1-89011] - Staining of human cerebral cortex shows positivity in neuronal cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Rhot1 Antibody [NBP1-89011] - Staining of human liver shows positivity in hepatocytes.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Rhot1 Antibody [NBP1-89011] - Staining of human pancreas shows positivity in exocrine glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Rhot1 Antibody [NBP1-89011] - Staining of human small intestine shows positivity in glandular cells.