HPA010687

antibody from Atlas Antibodies

Targeting: RHOT1 ARHT1, FLJ11040, MIRO-1, MIRO1

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA010687

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA010687, RRID:AB_1079813

Product name

Anti-RHOT1

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

THIVDYSEAEQSDEQLHQEISQANVICIVYAVNNK
HSIDKVTSRWIPLINERTDKDSRLPLILVGNKSDL
VEYSSMETILPIMNQYTEIE


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References

Submitted references

Miro1 Regulates Activity-Driven Positioning of Mitochondria within Astrocytic Processes Apposed to Synapses to Regulate Intracellular Calcium Signaling.

Stephen TL, Higgs NF, Sheehan DF, Al Awabdh S, López-Doménech G, Arancibia-Carcamo IL, Kittler JT
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Dec 2;35(48):15996-6011

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Mitotic redistribution of the mitochondrial network by Miro and Cenp-F.

Kanfer G, Courthéoux T, Peterka M, Meier S, Soste M, Melnik A, Reis K, Aspenström P, Peter M, Picotti P, Kornmann B
Nature communications 2015 Aug 11;6:8015

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Lysine 27 ubiquitination of the mitochondrial transport protein Miro is dependent on serine 65 of the Parkin ubiquitin ligase.

Birsa N, Norkett R, Wauer T, Mevissen TE, Wu HC, Foltynie T, Bhatia K, Hirst WD, Komander D, Plun-Favreau H, Kittler JT
The Journal of biological chemistry 2014 May 23;289(21):14569-82

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The mitochondrial deubiquitinase USP30 opposes parkin-mediated mitophagy

Bingol B, Tea J, Phu L, Reichelt M, Bakalarski C, Song Q, Foreman O, Kirkpatrick D, Sheng M
Nature 2014 June

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Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells

Stadler C, Rexhepaj E, Singan V, Murphy R, Pepperkok R, Uhlén M, Simpson J, Lundberg E
Nature Methods 2013 February;10(4):315-323

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VAPB interacts with the mitochondrial protein PTPIP51 to regulate calcium homeostasis

De Vos K, Morotz G, Stoica R, Tudor E, Lau K, Ackerley S, Warley A, Shaw C, Miller C
Human Molecular Genetics 2012 February;21(6):1299-1311

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human heart muscle shows strong granular cytoplasmic positivity in myocytes.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex shows positivity in neuronal cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebellum shows positivity in neuronal cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human small intestine shows positivity in glandular cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human liver shows positivity in hepatocytes.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human pancreas shows positivity in exocrine glandular cells.

Sample type

HUMAN