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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- NB100-56173 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56173, RRID:AB_839023
- Product name
- Rabbit Polyclonal TRAF-2 Antibody
- Antibody type
- Polyclonal
- Description
- Unpurified. The gene encoding TRAF2 produces, by alternative splicing, 15 different transcripts, all with introns, putatively encoding 15 different protein isoforms. These isoforms are of varying amino acid lengths. Therefore the molecular weight observed by western blot may vary, depending on the isoform expressed. Users are encouraged to use the peptide immunogen sequence information provided in the antigen section to gain additional information about the potential TRAF2 isoforms detected by the antibody.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.05 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Downregulation of TRAF2 mediates NIK-induced pancreatic cancer cell proliferation and tumorigenicity.
A novel dual signaling axis for NSP 5a3a induced apoptosis in head and neck carcinoma.
TNFR-associated factor family protein expression in normal tissues and lymphoid malignancies.
TNFR-associated factor family protein expression in normal tissues and lymphoid malignancies.
Döppler H, Liou GY, Storz P
PloS one 2013;8(1):e53676
PloS one 2013;8(1):e53676
A novel dual signaling axis for NSP 5a3a induced apoptosis in head and neck carcinoma.
D'Agostino L, Giordano A
Oncotarget 2011 Dec;2(12):1055-74
Oncotarget 2011 Dec;2(12):1055-74
TNFR-associated factor family protein expression in normal tissues and lymphoid malignancies.
Zapata JM, Krajewska M, Krajewski S, Kitada S, Welsh K, Monks A, McCloskey N, Gordon J, Kipps TJ, Gascoyne RD, Shabaik A, Reed JC
Journal of immunology (Baltimore, Md. : 1950) 2000 Nov 1;165(9):5084-96
Journal of immunology (Baltimore, Md. : 1950) 2000 Nov 1;165(9):5084-96
TNFR-associated factor family protein expression in normal tissues and lymphoid malignancies.
Zapata JM, Krajewska M, Krajewski S, Kitada S, Welsh K, Monks A, McCloskey N, Gordon J, Kipps TJ, Gascoyne RD, Shabaik A, Reed JC
Journal of immunology (Baltimore, Md. : 1950) 2000 Nov 1;165(9):5084-96
Journal of immunology (Baltimore, Md. : 1950) 2000 Nov 1;165(9):5084-96
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TRAF-2 Antibody - Unpurified [NB100-56173] - Detection of TRAF-2 protein in various tumor cell lines using this antibody at 1:2000 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TRAF-2 Antibody - Unpurified [NB100-56173] - Analysis of TRAF-2 in normal human tissues using this antibody. TRAF-2 is observed at ~60-64 kDa. Additional bands of lower molecular weight were seen in some cases, and may represent TRAF-2 degradation fragments.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TRAF-2 Antibody - Unpurified [NB100-56173] - Formalin-fixed, paraffin-embedded human colon carcinoma tissue sections stained for TRAF2 using this antibody at 1:2000. Hematoxylin-eosin counterstain.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: TRAF-2 Antibody - Unpurified [NB100-56173] - Formalin-fixed, paraffin-embedded human melanoma tissue section stained for TRAF2 at 1:2000. A1 is a higher magnification of A. Hematoxylin-eosin counterstain.
