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- Antigen structure
- References [26]
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- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- 18068-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#18068-1-AP, RRID:AB_2162931
- Product name
- PDH E1 Alpha antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated PDH E1 Alpha antibody (Cat. #18068-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references A novel regulatory mechanism of geniposide for improving glucose homeostasis mediated by circulating RBP4.
Lactic Acid Fermentation Is Required for NLRP3 Inflammasome Activation.
Growth hormone receptor disrupts glucose homeostasis via promoting and stabilizing retinol binding protein 4.
HIF-1 regulates energy metabolism of the Tibetan chicken brain during embryo development under hypoxia.
Localization of myoglobin in mitochondria: implication in regulation of mitochondrial respiration in rat skeletal muscle.
Resveratrol Is a Natural Inhibitor of Human Intestinal Mast Cell Activation and Phosphorylation of Mitochondrial ERK1/2 and STAT3.
Pyruvate-Enriched Oral Rehydration Solution Improves Glucometabolic Disorders in the Kidneys of Diabetic db/db Mice.
Fenofibrate-induced mitochondrial dysfunction and metabolic reprogramming reversal: the anti-tumor effects in gastric carcinoma cells mediated by the PPAR pathway.
A shift in glutamine nitrogen metabolism contributes to the malignant progression of cancer.
Spexin protects cardiomyocytes from hypoxia-induced metabolic and mitochondrial dysfunction.
Succinate dehydrogenase deficiency in a chromaffin cell model retains metabolic fitness through the maintenance of mitochondrial NADH oxidoreductase function.
The novel function of tumor protein D54 in regulating pyruvate dehydrogenase and metformin cytotoxicity in breast cancer.
ROS/KRAS/AMPK Signaling Contributes to Gemcitabine-Induced Stem-like Cell Properties in Pancreatic Cancer.
Low-dose PCB126 compromises circadian rhythms associated with disordered glucose and lipid metabolism in mice.
Dichloroacetic acid (DCA) synergizes with the SIRT2 inhibitor Sirtinol and AGK2 to enhance anti-tumor efficacy in non-small cell lung cancer.
Utilizing Optimized Tools to Investigate PTM Crosstalk: Identifying Potential PTM Crosstalk of Acetylated Mitochondrial Proteins.
(Pro)renin Receptor Inhibition Reprograms Hepatic Lipid Metabolism and Protects Mice From Diet-Induced Obesity and Hepatosteatosis.
RIP3 targets pyruvate dehydrogenase complex to increase aerobic respiration in TNF-induced necroptosis.
Exogenous H2S switches cardiac energy substrate metabolism by regulating SIRT3 expression in db/db mice.
The microRNA-182-PDK4 axis regulates lung tumorigenesis by modulating pyruvate dehydrogenase and lipogenesis.
Redox homeostasis protects mitochondria through accelerating ROS conversion to enhance hypoxia resistance in cancer cells.
Environment Impacts the Metabolic Dependencies of Ras-Driven Non-Small Cell Lung Cancer.
ROS-mediated glucose metabolic reprogram induces insulin resistance in type 2 diabetes.
The oncoprotein HBXIP promotes glucose metabolism reprogramming via downregulating SCO2 and PDHA1 in breast cancer.
Asparagine plays a critical role in regulating cellular adaptation to glutamine depletion.
Mitochondrial bioenergetic deficits in the hippocampi of rats with chronic ischemia-induced vascular dementia.
Liu J, Song C, Nie C, Sun Y, Wang Y, Xue L, Fan M, Qian H, Wang L, Li Y
Phytomedicine : international journal of phytotherapy and phytopharmacology 2022 Jan;95:153862
Phytomedicine : international journal of phytotherapy and phytopharmacology 2022 Jan;95:153862
Lactic Acid Fermentation Is Required for NLRP3 Inflammasome Activation.
Lin HC, Chen YJ, Wei YH, Lin HA, Chen CC, Liu TF, Hsieh YL, Huang KY, Lin KH, Wang HH, Chen LC
Frontiers in immunology 2021;12:630380
Frontiers in immunology 2021;12:630380
Growth hormone receptor disrupts glucose homeostasis via promoting and stabilizing retinol binding protein 4.
Liu J, Nie C, Xue L, Yan Y, Liu S, Sun J, Fan M, Qian H, Ying H, Wang L, Li Y
Theranostics 2021;11(17):8283-8300
Theranostics 2021;11(17):8283-8300
HIF-1 regulates energy metabolism of the Tibetan chicken brain during embryo development under hypoxia.
Tang Q, Xu Q, Ding C, Zhang H, Ling Y, Wu C, Fang M
American journal of physiology. Regulatory, integrative and comparative physiology 2021 May 1;320(5):R704-R713
American journal of physiology. Regulatory, integrative and comparative physiology 2021 May 1;320(5):R704-R713
Localization of myoglobin in mitochondria: implication in regulation of mitochondrial respiration in rat skeletal muscle.
Koma R, Shibaguchi T, Pérez López C, Oka T, Jue T, Takakura H, Masuda K
Physiological reports 2021 Mar;9(5):e14769
Physiological reports 2021 Mar;9(5):e14769
Resveratrol Is a Natural Inhibitor of Human Intestinal Mast Cell Activation and Phosphorylation of Mitochondrial ERK1/2 and STAT3.
Bilotta S, Paruchuru LB, Feilhauer K, Köninger J, Lorentz A
International journal of molecular sciences 2021 Jul 16;22(14)
International journal of molecular sciences 2021 Jul 16;22(14)
Pyruvate-Enriched Oral Rehydration Solution Improves Glucometabolic Disorders in the Kidneys of Diabetic db/db Mice.
Zhang XM, Deng H, Tong JD, Wang YZ, Ning XC, Yang XH, Zhou FQ, Jin HM
Journal of diabetes research 2020;2020:2817972
Journal of diabetes research 2020;2020:2817972
Fenofibrate-induced mitochondrial dysfunction and metabolic reprogramming reversal: the anti-tumor effects in gastric carcinoma cells mediated by the PPAR pathway.
Chen L, Peng J, Wang Y, Jiang H, Wang W, Dai J, Tang M, Wei Y, Kuang H, Xu G, Xu H, Zhou F
American journal of translational research 2020;12(2):428-446
American journal of translational research 2020;12(2):428-446
A shift in glutamine nitrogen metabolism contributes to the malignant progression of cancer.
Kodama M, Oshikawa K, Shimizu H, Yoshioka S, Takahashi M, Izumi Y, Bamba T, Tateishi C, Tomonaga T, Matsumoto M, Nakayama KI
Nature communications 2020 Mar 17;11(1):1320
Nature communications 2020 Mar 17;11(1):1320
Spexin protects cardiomyocytes from hypoxia-induced metabolic and mitochondrial dysfunction.
Liu Y, Sun L, Zheng L, Su M, Liu H, Wei Y, Li D, Wang Y, Dai C, Gong Y, Zhao C, Li Y
Naunyn-Schmiedeberg's archives of pharmacology 2020 Jan;393(1):25-33
Naunyn-Schmiedeberg's archives of pharmacology 2020 Jan;393(1):25-33
Succinate dehydrogenase deficiency in a chromaffin cell model retains metabolic fitness through the maintenance of mitochondrial NADH oxidoreductase function.
Kľučková K, Thakker A, Vettore L, Escribano-Gonzalez C, Hindshaw RL, Tearle JLE, Goncalves J, Kaul B, Lavery GG, Favier J, Tennant DA
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Jan;34(1):303-315
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Jan;34(1):303-315
The novel function of tumor protein D54 in regulating pyruvate dehydrogenase and metformin cytotoxicity in breast cancer.
Zhuang Y, Ly RC, Frazier CV, Yu J, Qin S, Fan XY, Goetz MP, Boughey JC, Weinshilboum R, Wang L
Cancer & metabolism 2019;7:1
Cancer & metabolism 2019;7:1
ROS/KRAS/AMPK Signaling Contributes to Gemcitabine-Induced Stem-like Cell Properties in Pancreatic Cancer.
Zhao H, Wu S, Li H, Duan Q, Zhang Z, Shen Q, Wang C, Yin T
Molecular therapy oncolytics 2019 Sep 27;14:299-312
Molecular therapy oncolytics 2019 Sep 27;14:299-312
Low-dose PCB126 compromises circadian rhythms associated with disordered glucose and lipid metabolism in mice.
Shen X, Chen Y, Zhang J, Yan X, Liu W, Guo Y, Shan Q, Liu S
Environment international 2019 Jul;128:146-157
Environment international 2019 Jul;128:146-157
Dichloroacetic acid (DCA) synergizes with the SIRT2 inhibitor Sirtinol and AGK2 to enhance anti-tumor efficacy in non-small cell lung cancer.
Ma W, Zhao X, Wang K, Liu J, Huang G
Cancer biology & therapy 2018;19(9):835-846
Cancer biology & therapy 2018;19(9):835-846
Utilizing Optimized Tools to Investigate PTM Crosstalk: Identifying Potential PTM Crosstalk of Acetylated Mitochondrial Proteins.
Horita H, Law A, Middleton K
Proteomes 2018 May 22;6(2)
Proteomes 2018 May 22;6(2)
(Pro)renin Receptor Inhibition Reprograms Hepatic Lipid Metabolism and Protects Mice From Diet-Induced Obesity and Hepatosteatosis.
Ren L, Sun Y, Lu H, Ye D, Han L, Wang N, Daugherty A, Li F, Wang M, Su F, Tao W, Sun J, Zelcer N, Mullick AE, Danser AHJ, Jiang Y, He Y, Ruan X, Lu X
Circulation research 2018 Mar 2;122(5):730-741
Circulation research 2018 Mar 2;122(5):730-741
RIP3 targets pyruvate dehydrogenase complex to increase aerobic respiration in TNF-induced necroptosis.
Yang Z, Wang Y, Zhang Y, He X, Zhong CQ, Ni H, Chen X, Liang Y, Wu J, Zhao S, Zhou D, Han J
Nature cell biology 2018 Feb;20(2):186-197
Nature cell biology 2018 Feb;20(2):186-197
Exogenous H2S switches cardiac energy substrate metabolism by regulating SIRT3 expression in db/db mice.
Sun Y, Tian Z, Liu N, Zhang L, Gao Z, Sun X, Yu M, Wu J, Yang F, Zhao Y, Ren H, Chen H, Zhao D, Wang Y, Dong S, Xu C, Lu F, Zhang W
Journal of molecular medicine (Berlin, Germany) 2018 Apr;96(3-4):281-299
Journal of molecular medicine (Berlin, Germany) 2018 Apr;96(3-4):281-299
The microRNA-182-PDK4 axis regulates lung tumorigenesis by modulating pyruvate dehydrogenase and lipogenesis.
Li G, Li M, Hu J, Lei R, Xiong H, Ji H, Yin H, Wei Q, Hu G
Oncogene 2017 Feb 16;36(7):989-998
Oncogene 2017 Feb 16;36(7):989-998
Redox homeostasis protects mitochondria through accelerating ROS conversion to enhance hypoxia resistance in cancer cells.
Li P, Zhang D, Shen L, Dong K, Wu M, Ou Z, Shi D
Scientific reports 2016 Mar 9;6:22831
Scientific reports 2016 Mar 9;6:22831
Environment Impacts the Metabolic Dependencies of Ras-Driven Non-Small Cell Lung Cancer.
Davidson SM, Papagiannakopoulos T, Olenchock BA, Heyman JE, Keibler MA, Luengo A, Bauer MR, Jha AK, O'Brien JP, Pierce KA, Gui DY, Sullivan LB, Wasylenko TM, Subbaraj L, Chin CR, Stephanopolous G, Mott BT, Jacks T, Clish CB, Vander Heiden MG
Cell metabolism 2016 Mar 8;23(3):517-28
Cell metabolism 2016 Mar 8;23(3):517-28
ROS-mediated glucose metabolic reprogram induces insulin resistance in type 2 diabetes.
Dong K, Ni H, Wu M, Tang Z, Halim M, Shi D
Biochemical and biophysical research communications 2016 Aug 5;476(4):204-211
Biochemical and biophysical research communications 2016 Aug 5;476(4):204-211
The oncoprotein HBXIP promotes glucose metabolism reprogramming via downregulating SCO2 and PDHA1 in breast cancer.
Liu F, Zhang W, You X, Liu Y, Li Y, Wang Z, Wang Y, Zhang X, Ye L
Oncotarget 2015 Sep 29;6(29):27199-213
Oncotarget 2015 Sep 29;6(29):27199-213
Asparagine plays a critical role in regulating cellular adaptation to glutamine depletion.
Zhang J, Fan J, Venneti S, Cross JR, Takagi T, Bhinder B, Djaballah H, Kanai M, Cheng EH, Judkins AR, Pawel B, Baggs J, Cherry S, Rabinowitz JD, Thompson CB
Molecular cell 2014 Oct 23;56(2):205-218
Molecular cell 2014 Oct 23;56(2):205-218
Mitochondrial bioenergetic deficits in the hippocampi of rats with chronic ischemia-induced vascular dementia.
Du J, Ma M, Zhao Q, Fang L, Chang J, Wang Y, Fei R, Song X
Neuroscience 2013 Feb 12;231:345-52
Neuroscience 2013 Feb 12;231:345-52
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- mouse brain tissue were subjected to SDS PAGE followed by western blot with 18068-1-AP(PDHA1 antibody) at dilution of 1:500
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using PDHA1 antibody 18068-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human heart using 18068-1-AP(PDHA1 antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human heart using 18068-1-AP(PDHA1 antibody) at dilution of 1:100 (under 40x lens)
- Sample type
- tissue
