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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- ARG54014 - Provider product page
- Provider
- Arigo
- Product name
- anti-Pyruvate Dehydrogenase E1-alpha subunit antibody
- Antibody type
- Monoclonal
- Antigen
- Purified recombinant human Pyruvate Dehydrogenase protein fragments expressed in E.coli.
- Description
- Affinity purified
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3H2-F8-B5
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
- Storage
- Aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: pyruvate dehydrogenase (lipoamide) alpha 1 in 293, 1299, mEsc and Tc1 cell lysates stained with Pyruvate Dehydrogenase E1-alpha subunit antibody [3H2-F8-B5] (ARG54014) (diluted in 1: 1000). Predicted band size: 43KDa. Observed band size: 43KDa.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: HeLa cells fixed with 4% Paraformaldehyde and stained with Pyruvate Dehydrogenase E1-alpha subunit antibody [3H2-F8-B5] (ARG54014) (diluted in 1:100).
