TA502696

antibody from OriGene

Targeting: PDHA1 PDHA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA502696

Provider

OriGene

Proper citation

OriGene Cat#TA502696, RRID:AB_11126173

Product name

PDHA1 mouse monoclonal antibody, clone OTI2C10 (formerly 2C10)

Antibody type

Monoclonal

Description

PDHA1 mouse monoclonal antibody, clone OTI2C10 (formerly 2C10)

Host

Mouse

Conjugate

Unconjugated

Epitope

PDHA1

Isotype

IgG

Antibody clone number

OTI2C10

Vial size

100 µl

Concentration

0.75 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDHA1 (RC201831, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDHA1.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-PDHA1 mouse monoclonal antibody (TA502696) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDHA1(RC201831).

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of Hela cells, using anti-PDHA1 antibody(TA502696),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

Validation comment

FC