Antibody data
- Antibody Data
- Antigen structure
- References [19]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- AF2148 - Provider product page
- Provider
- R&D Systems
- Product name
- Human LDLR Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human LDL R in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant mouse LDL R is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P01130
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references HIV-induced neuroinflammation: impact of PAR1 and PAR2 processing by Furin.
SR-B1 drives endothelial cell LDL transcytosis via DOCK4 to promote atherosclerosis.
The ER membrane protein complex promotes biogenesis of sterol-related enzymes maintaining cholesterol homeostasis.
6-Gingerol Regulates Hepatic Cholesterol Metabolism by Up-regulation of LDLR and Cholesterol Efflux-Related Genes in HepG2 Cells.
Low-density lipoprotein (LDL)-dependent uptake of Gram-positive lipoteichoic acid and Gram-negative lipopolysaccharide occurs through LDL receptor.
Antisense oligonucleotides targeting translation inhibitory elements in 5' UTRs can selectively increase protein levels.
Applying antibody-sensitive hypervariable region 1-deleted hepatitis C virus to the study of escape pathways of neutralizing human monoclonal antibody AR5A.
The Proprotein Convertase Subtilisin/Kexin Type 9-resistant R410S Low Density Lipoprotein Receptor Mutation: A NOVEL MECHANISM CAUSING FAMILIAL HYPERCHOLESTEROLEMIA.
Ruxolitinib and Polycation Combination Treatment Overcomes Multiple Mechanisms of Resistance of Pancreatic Cancer Cells to Oncolytic Vesicular Stomatitis Virus.
Identification of a Chrysanthemic Ester as an Apolipoprotein E Inducer in Astrocytes.
Alirocumab, a Therapeutic Human Antibody to PCSK9, Does Not Affect CD81 Levels or Hepatitis C Virus Entry and Replication into Hepatocytes.
Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) Single Domain Antibodies Are Potent Inhibitors of Low Density Lipoprotein Receptor Degradation.
Common Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) Epitopes Mediate Multiple Routes for Internalization and Function.
Plasma Membrane Tetraspanin CD81 Complexes with Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) and Low Density Lipoprotein Receptor (LDLR), and Its Levels Are Reduced by PCSK9.
Differential complement activation pathways promote C3b deposition on native and acetylated LDL thereby inducing lipoprotein binding to the complement receptor 1.
Hormonal modulators of glial ABCA1 and apoE levels.
Characterization of proprotein convertase subtilisin/kexin type 9 (PCSK9) trafficking reveals a novel lysosomal targeting mechanism via amyloid precursor-like protein 2 (APLP2).
The proprotein convertase PC7: unique zymogen activation and trafficking pathways.
Dissection of the endogenous cellular pathways of PCSK9-induced low density lipoprotein receptor degradation: evidence for an intracellular route.
Sachan V, Lodge R, Mihara K, Hamelin J, Power C, Gelman BB, Hollenberg MD, Cohen ÉA, Seidah NG
Cell death and differentiation 2019 Oct;26(10):1942-1954
Cell death and differentiation 2019 Oct;26(10):1942-1954
SR-B1 drives endothelial cell LDL transcytosis via DOCK4 to promote atherosclerosis.
Huang L, Chambliss KL, Gao X, Yuhanna IS, Behling-Kelly E, Bergaya S, Ahmed M, Michaely P, Luby-Phelps K, Darehshouri A, Xu L, Fisher EA, Ge WP, Mineo C, Shaul PW
Nature 2019 May;569(7757):565-569
Nature 2019 May;569(7757):565-569
The ER membrane protein complex promotes biogenesis of sterol-related enzymes maintaining cholesterol homeostasis.
Volkmar N, Thezenas ML, Louie SM, Juszkiewicz S, Nomura DK, Hegde RS, Kessler BM, Christianson JC
Journal of cell science 2019 Jan 16;132(2)
Journal of cell science 2019 Jan 16;132(2)
6-Gingerol Regulates Hepatic Cholesterol Metabolism by Up-regulation of LDLR and Cholesterol Efflux-Related Genes in HepG2 Cells.
Li X, Guo J, Liang N, Jiang X, Song Y, Ou S, Hu Y, Jiao R, Bai W
Frontiers in pharmacology 2018;9:159
Frontiers in pharmacology 2018;9:159
Low-density lipoprotein (LDL)-dependent uptake of Gram-positive lipoteichoic acid and Gram-negative lipopolysaccharide occurs through LDL receptor.
Grin PM, Dwivedi DJ, Chathely KM, Trigatti BL, Prat A, Seidah NG, Liaw PC, Fox-Robichaud AE
Scientific reports 2018 Jul 12;8(1):10496
Scientific reports 2018 Jul 12;8(1):10496
Antisense oligonucleotides targeting translation inhibitory elements in 5' UTRs can selectively increase protein levels.
Liang XH, Sun H, Shen W, Wang S, Yao J, Migawa MT, Bui HH, Damle SS, Riney S, Graham MJ, Crooke RM, Crooke ST
Nucleic acids research 2017 Sep 19;45(16):9528-9546
Nucleic acids research 2017 Sep 19;45(16):9528-9546
Applying antibody-sensitive hypervariable region 1-deleted hepatitis C virus to the study of escape pathways of neutralizing human monoclonal antibody AR5A.
Velázquez-Moctezuma R, Law M, Bukh J, Prentoe J
PLoS pathogens 2017 Feb;13(2):e1006214
PLoS pathogens 2017 Feb;13(2):e1006214
The Proprotein Convertase Subtilisin/Kexin Type 9-resistant R410S Low Density Lipoprotein Receptor Mutation: A NOVEL MECHANISM CAUSING FAMILIAL HYPERCHOLESTEROLEMIA.
Susan-Resiga D, Girard E, Kiss RS, Essalmani R, Hamelin J, Asselin MC, Awan Z, Butkinaree C, Fleury A, Soldera A, Dory YL, Baass A, Seidah NG
The Journal of biological chemistry 2017 Feb 3;292(5):1573-1590
The Journal of biological chemistry 2017 Feb 3;292(5):1573-1590
Ruxolitinib and Polycation Combination Treatment Overcomes Multiple Mechanisms of Resistance of Pancreatic Cancer Cells to Oncolytic Vesicular Stomatitis Virus.
Felt SA, Droby GN, Grdzelishvili VZ
Journal of virology 2017 Aug 15;91(16)
Journal of virology 2017 Aug 15;91(16)
Identification of a Chrysanthemic Ester as an Apolipoprotein E Inducer in Astrocytes.
Fan J, Zareyan S, Zhao W, Shimizu Y, Pfeifer TA, Tak JH, Isman MB, Van den Hoven B, Duggan ME, Wood MW, Wellington CL, Kulic I
PloS one 2016;11(9):e0162384
PloS one 2016;11(9):e0162384
Alirocumab, a Therapeutic Human Antibody to PCSK9, Does Not Affect CD81 Levels or Hepatitis C Virus Entry and Replication into Hepatocytes.
Ramanathan A, Gusarova V, Stahl N, Gurnett-Bander A, Kyratsous CA
PloS one 2016;11(4):e0154498
PloS one 2016;11(4):e0154498
Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) Single Domain Antibodies Are Potent Inhibitors of Low Density Lipoprotein Receptor Degradation.
Weider E, Susan-Resiga D, Essalmani R, Hamelin J, Asselin MC, Nimesh S, Ashraf Y, Wycoff KL, Zhang J, Prat A, Seidah NG
The Journal of biological chemistry 2016 Aug 5;291(32):16659-71
The Journal of biological chemistry 2016 Aug 5;291(32):16659-71
Common Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) Epitopes Mediate Multiple Routes for Internalization and Function.
DeVay RM, Yamamoto L, Shelton DL, Liang H
PloS one 2015;10(4):e0125127
PloS one 2015;10(4):e0125127
Plasma Membrane Tetraspanin CD81 Complexes with Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) and Low Density Lipoprotein Receptor (LDLR), and Its Levels Are Reduced by PCSK9.
Le QT, Blanchet M, Seidah NG, Labonté P
The Journal of biological chemistry 2015 Sep 18;290(38):23385-400
The Journal of biological chemistry 2015 Sep 18;290(38):23385-400
Differential complement activation pathways promote C3b deposition on native and acetylated LDL thereby inducing lipoprotein binding to the complement receptor 1.
Klop B, van der Pol P, van Bruggen R, Wang Y, de Vries MA, van Santen S, O'Flynn J, van de Geijn GJ, Njo TL, Janssen HW, de Man P, Jukema JW, Rabelink TJ, Rensen PC, van Kooten C, Cabezas MC
The Journal of biological chemistry 2014 Dec 19;289(51):35421-30
The Journal of biological chemistry 2014 Dec 19;289(51):35421-30
Hormonal modulators of glial ABCA1 and apoE levels.
Fan J, Shimizu Y, Chan J, Wilkinson A, Ito A, Tontonoz P, Dullaghan E, Galea LA, Pfeifer T, Wellington CL
Journal of lipid research 2013 Nov;54(11):3139-50
Journal of lipid research 2013 Nov;54(11):3139-50
Characterization of proprotein convertase subtilisin/kexin type 9 (PCSK9) trafficking reveals a novel lysosomal targeting mechanism via amyloid precursor-like protein 2 (APLP2).
DeVay RM, Shelton DL, Liang H
The Journal of biological chemistry 2013 Apr 12;288(15):10805-18
The Journal of biological chemistry 2013 Apr 12;288(15):10805-18
The proprotein convertase PC7: unique zymogen activation and trafficking pathways.
Rousselet E, Benjannet S, Hamelin J, Canuel M, Seidah NG
The Journal of biological chemistry 2011 Jan 28;286(4):2728-38
The Journal of biological chemistry 2011 Jan 28;286(4):2728-38
Dissection of the endogenous cellular pathways of PCSK9-induced low density lipoprotein receptor degradation: evidence for an intracellular route.
Poirier S, Mayer G, Poupon V, McPherson PS, Desjardins R, Ly K, Asselin MC, Day R, Duclos FJ, Witmer M, Parker R, Prat A, Seidah NG
The Journal of biological chemistry 2009 Oct 16;284(42):28856-64
The Journal of biological chemistry 2009 Oct 16;284(42):28856-64
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- LDL R in HepG2 Human Cell Line. LDL R was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human LDL R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2148) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- LDL R in Human Liver. LDL R was detected in formalin fixed paraffin-embedded sections of human liver using Goat Anti-Human LDL R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2148) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.