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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- GTX31957 - Provider product page
- Provider
- GeneTex
- Product name
- K-RAS antibody [AT2F8]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse
- Host
- Mouse
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- The Cell lysates (40μg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRAS antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjμgated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : HepG2 cell lysate Lane 3. : Ramos cell lysate Lane 4. : A549 cell lysate Lane 5. : Balb/3T3 cell lysate
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ICC/IF analysis of KRAS in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human KRAS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjμgate (Green).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flow cytometry analysis of KRAS in A549 cell line, staining at 2-5μg for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjμgate. Isotype control antibody was mouse IgG (black line).
