Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AM50616PU-N - Provider product page
- Provider
- OriGene
- Product name
- KRAS mouse monoclonal antibody, clone AT2F8, Purified
- Antibody type
- Monoclonal
- Description
- KRAS mouse monoclonal antibody, clone AT2F8, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- KRAS
- Antibody clone number
- AT2F8
- Vial size
- 100 µl
- Concentration
- 1.0 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRAS antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : HepG2 cell lysate Lane 3. : Ramos cell lysate Lane 4. : A549 cell lysate Lane 5. : Balb/3T3 cell lysate
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- ICC/IF analysis of KRAS in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human KRAS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
- Validation comment
- IF
